Molecular Survey of Hemotropic Mycoplasma spp. and Bartonella spp. in Coatis (Nasua nasua) from Central-Western Brazil

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dc.contributor.authorPerles, Lívia [UNESP]
dc.contributor.authorBarreto, Wanessa Teixeira Gomes
dc.contributor.authorSantos, Filipe Martins
dc.contributor.authorDuarte, Leidiane Lima
dc.contributor.authorde Macedo, Gabriel Carvalho
dc.contributor.authorBarros-Battesti, Darci Moraes [UNESP]
dc.contributor.authorHerrera, Heitor Miraglia
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionMato Grosso do Sul Federal University
dc.contributor.institutionDom Bosco Catholic University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T16:12:52Z
dc.date.available2023-07-29T16:12:52Z
dc.date.issued2023-04-01
dc.description.abstractEven though previous works showed molecular evidence of hemotropic Mycoplasma spp. (hemoplasmas) in ring-tailed coatis (Nasua nasua) from Brazil, Bartonella sp. has not been reported in these mammals so far. The present study aimed to detect the above-mentioned agents in coatis’ blood and associated ectoparasites, assessing the association between these infections and red blood parameters. Between March 2018 and January 2019, coati (n = 97) blood samples, Amblyomma sp. ticks (2242 individual ticks, resulting in 265 pools), and Neotrichodectes pallidus louse (n = 59) were collected in forested urban areas from midwestern Brazil. DNA extracted from coatis’ blood, and ectoparasite samples were submitted to quantitative PCR (qPCR) (16S rRNA) and conventional PCR (cPCR) (16S rRNA and 23S rRNA) for hemoplasmas and qPCR (nuoG gene) and culturing (only blood) for Bartonella spp. Two different hemoplasma genotypes were detected in blood samples: 71% coatis positive for myc1 and 17% positive for myc2. While 10% of ticks were positive for hemoplasmas (myc1), no louse was positive. The estimated bacterial load of hemoplasmas showed no association with anemia indicators. All coatis were negative for Bartonella sp. in qPCR assay and culturing, albeit two Amblyomma sp. larvae pools, and 2 A. dubitatum nymph pools were positive in the qPCR. The present work showed a high occurrence of hemoplasmas, with two distinct hemoplasma genotypes, in coatis from forested urban areas in midwestern Brazil.en
dc.description.affiliationVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural
dc.description.affiliationPost-Graduation of Ecology and Conservation Mato Grosso do Sul Federal University
dc.description.affiliationLaboratory of Parasitic Biology Environmental Sciences and Farming Sustainability Dom Bosco Catholic University
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Science School of Veterinary Medicine University of São Paulo
dc.description.affiliationUnespVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipHackensack Meridian Health
dc.description.sponsorshipIdFAPESP: 2018/02753-0
dc.description.sponsorshipIdFAPESP: 2019/15150-4
dc.description.sponsorshipIdFAPESP: 2020/12037-0
dc.description.sponsorshipIdCNPq: 303701/2021-8
dc.description.sponsorshipIdHackensack Meridian Health: 308768/2017-5
dc.identifierhttp://dx.doi.org/10.3390/pathogens12040538
dc.identifier.citationPathogens, v. 12, n. 4, 2023.
dc.identifier.doi10.3390/pathogens12040538
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85156101344
dc.identifier.urihttp://hdl.handle.net/11449/249919
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subjectAmblyommaspp
dc.subjectbartonellae
dc.subjecthemotropic Mycoplasmaspp
dc.subjectmidwestern Brazil
dc.subjectNeotrichodectes pallidus
dc.titleMolecular Survey of Hemotropic Mycoplasma spp. and Bartonella spp. in Coatis (Nasua nasua) from Central-Western Brazilen
dc.typeArtigo
unesp.author.orcid0000-0003-2032-8129[3]
unesp.author.orcid0000-0003-2404-8765[7]
unesp.author.orcid0000-0002-1713-5222[9]
unesp.departmentPatologia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias