Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production

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dc.contributor.authorBotigelli, Ramon Cesar
dc.contributor.authorSchwarz, Katia Lancellotti
dc.contributor.authorZaffalon, Fabiane Gilli
dc.contributor.authorDel Collado, Maite
dc.contributor.authorCastro, Fernanda Cavallari
dc.contributor.authorFernandes, Hugo
dc.contributor.authorLeal, Claudia Lima Verde
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Do Estado de São Paulo
dc.date.accessioned2022-04-29T08:45:17Z
dc.date.available2022-04-29T08:45:17Z
dc.date.issued2017-06-01
dc.description.abstractThis study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78.4 ± 2.4% for controls, P < 0.05) and ODQ reversed its effect (73.4 ± 6.3%, P > 0.05). Cyclic GMP levels were higher in SNAP (3.94 ± 0.18, P < 0.05) and ODQ abolished the effect (2.48 ± 0.13 pmol/COC, P < 0.05), while cAMP levels were decreased in both treatments. Experiment II, COCs were cultured with SNAP alone or with PDEs inhibitors. SNAP alone or with PDEs inhibitors delayed GVBD (24.7 ± 2.8 to 56.9 ± 8.7%, P < 0.05) compared with the control (77.1 ± 1.8%), and SNAP and SNAP + cilostamide had lowest rates (34.9 ± 9.2% and 24.7 ± 2.8%). Experiment III, COCs were cultured (24-28 h) with SNAP and SNAP + cilostamide to assess metaphase II (MII) rates and embryo production. SNAP + cilostamide (50.0 ± 2.0%, P < 0.05) had lower MII rates at 24 h in vitro maturation (IVM), but at 28 h all groups were similar (66.6 to 71.4%, P > 0.05). Embryo development did not differ from the control for SNAP and cilostamide groups (38.7 ± 5.8, 37.9 ± 6.2 and 40.5 ± 5.8%, P > 0.05), but SNAP + cilostamide decreased embryo production (25.7 ± 6.9%, P < 0.05). In conclusion, SNAP was confirmed to delay meiosis resumption by the NO/sGC/cGMP pathway, by increasing cGMP, but not cAMP. Inhibiting different PDEs to further increase nucleotides in association with SNAP did not show any additive effects on meiosis resumption, indicating that other pathways are involved. Moreover, SNAP + cilostamide affected the meiosis progression and decreased embryo development.en
dc.description.affiliationDepartamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo, Av. Duque de Caxias Norte, 225
dc.description.affiliationDepartamento de Farmacologia Instituto de Biociências de Botucatu Universidade Do Estado de São Paulo, Distrito de Rubião Junior
dc.format.extent321-330
dc.identifierhttp://dx.doi.org/10.1017/S096719941700017X
dc.identifier.citationZygote, v. 25, n. 3, p. 321-330, 2017.
dc.identifier.doi10.1017/S096719941700017X
dc.identifier.issn1469-8730
dc.identifier.issn0967-1994
dc.identifier.scopus2-s2.0-85021254981
dc.identifier.urihttp://hdl.handle.net/11449/231403
dc.language.isoeng
dc.relation.ispartofZygote
dc.sourceScopus
dc.subjectcAMP
dc.subjectCattle
dc.subjectcGMP
dc.subjectCyclic nucleotides
dc.subjectSoluble guanylate cyclase
dc.titleInfluence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo productionen
dc.typeArtigo

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