Applicability of the SNPforID 52-plex panel for human identification and ancestry evaluation in a Brazilian population sample by next-generation sequencing

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dc.contributor.authorValle-Silva, Guilherme do
dc.contributor.authorSouza, Flávia Djenane Nunes de
dc.contributor.authorMarcorin, Letícia
dc.contributor.authorPereira, Alison Luis Eburneo
dc.contributor.authorCarratto, Thássia Mayra Telles
dc.contributor.authorDebortoli, Guilherme
dc.contributor.authorOliveira, Maria Luiza Guimarães de
dc.contributor.authorFracasso, Nádia Carolina de Aguiar
dc.contributor.authorAndrade, Edilene Santos de
dc.contributor.authorDonadi, Eduardo Antônio
dc.contributor.authorNorton, Heather L.
dc.contributor.authorParra, Esteban J.
dc.contributor.authorSimões, Aguinaldo Luiz
dc.contributor.authorCastelli, Erick C. [UNESP]
dc.contributor.authorMendes-Junior, Celso Teixeira
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Toronto at Mississauga
dc.contributor.institutionBarretos Cancer Hospital
dc.contributor.institutionUniversity of Cincinnati
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:49:19Z
dc.date.available2019-10-06T16:49:19Z
dc.date.issued2019-05-01
dc.description.abstractSNP analysis is of paramount importance in forensic genetics. The development of new technologies in next-generation sequencing allowed processing a large number of markers in various samples simultaneously. Although SNPs are less informative than STRs, they present lower mutation rates and perform better when using degraded samples. Some SNP systems were developed for forensic usage, such as the SNPforID 52-plex, from the SNPforID Consortium, containing 52 bi-allelic SNPs for human identification. In this paper we evaluated the informativeness of this system in a Brazilian population sample (n = 340). DNA libraries were prepared using a customized HaloPlex Target Enrichment System kit (Agilent Technologies, Inc.) and sequenced in the MiSeq Personal Sequencer platform (Illumina Inc.). The methodology presented here allowed the analysis of 51 out of 52 SNPforID markers. Allele frequencies and forensic parameters were estimated, revealing high informativeness: the combined match probability and power of exclusion were 6.48 × 10 −21 and 0.9997, respectively. Population admixture analysis indicates high European contribution (more than 70%) and low Amerindian contribution (less than 10%) in our population, while individual admixture analyses were consistent with the majority of individuals presenting high European contribution. This study demonstrates that the 52-plex kit is suitable for forensic cases in a Brazilian population, presenting results comparable with those obtained using a 16 STR panel.en
dc.description.affiliationDepartamento de Química Laboratório de Pesquisas Forenses e Genômicas Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartment of Anthropology University of Toronto at Mississauga
dc.description.affiliationMolecular Oncology Research Center Barretos Cancer Hospital
dc.description.affiliationDivisão de Imunologia Clínica Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartment of Anthropology University of Cincinnati
dc.description.affiliationSão Paulo State University (UNESP) Department of Pathology School of Medicine
dc.description.affiliationUnespSão Paulo State University (UNESP) Department of Pathology School of Medicine
dc.description.sponsorshipNational Institute of Justice
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Goiás
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipIdNational Institute of Justice: # 2013-DN-BX-K011
dc.description.sponsorshipIdFundação de Amparo à Pesquisa do Estado de Goiás: #2013/15447-0
dc.description.sponsorshipIdCAPES: #302590/2016-1
dc.description.sponsorshipIdCNPq: #448242/2014-1
dc.description.sponsorshipIdCAPES: (#304931/2014-1
dc.description.sponsorshipIdCAPES: (#309572/2014-2
dc.format.extent201-209
dc.identifierhttp://dx.doi.org/10.1016/j.fsigen.2019.03.003
dc.identifier.citationForensic Science International: Genetics, v. 40, p. 201-209.
dc.identifier.doi10.1016/j.fsigen.2019.03.003
dc.identifier.issn1878-0326
dc.identifier.issn1872-4973
dc.identifier.scopus2-s2.0-85062916063
dc.identifier.urihttp://hdl.handle.net/11449/189698
dc.language.isoeng
dc.relation.ispartofForensic Science International: Genetics
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBrazil
dc.subjectForensic genetics
dc.subjectGenetic admixture
dc.subjectMassively-parallel sequencing
dc.subjectSNPforID 52-plex
dc.subjectSNPs
dc.titleApplicability of the SNPforID 52-plex panel for human identification and ancestry evaluation in a Brazilian population sample by next-generation sequencingen
dc.typeArtigo
unesp.author.orcid0000-0001-9630-898X[3]
unesp.author.orcid0000-0003-4320-5850 0000-0003-4320-5850[6]
unesp.author.orcid0000-0003-2142-7196[14]
unesp.author.orcid0000-0002-7337-1203[15]

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