Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study

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dc.contributor.authorNai, Gisele Alborghetti
dc.contributor.authorMedina, Denis Aloísio Lopes
dc.contributor.authorMartelli, Cesar Alberto Talavera
dc.contributor.authorde Oliveira, Mayla Silva Cayres
dc.contributor.authorPortelinha, Maria Júlia Schadeck
dc.contributor.authorHenriques, Bruno Carvalho
dc.contributor.authorCaldeira, Isadora Delfino
dc.contributor.authorAlmeida, Mércia de Carvalho
dc.contributor.authorEller, Lizziane Kretli Winkelstroter
dc.contributor.authorNeto, Fausto Viterbo de Oliveira
dc.contributor.authorMarques, Mariângela Esther Alencar [UNESP]
dc.contributor.institutionGraduate Program in Health Sciences
dc.contributor.institutionGraduate Program in Animal Science
dc.contributor.institutionSchool of Medicine
dc.contributor.institutionLaboratory of Clinical Analysis
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:57:26Z
dc.date.available2021-06-25T10:57:26Z
dc.date.issued2021-04-02
dc.description.abstractABSTRACT: Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination.The following microorganisms were evaluated: Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Enterococcus faecalis. Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool.The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses.Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis, in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.en
dc.description.affiliationDepartment of Pathology
dc.description.affiliationGraduate Program in Health Sciences
dc.description.affiliationGraduate Program in Animal Science
dc.description.affiliationSchool of Medicine
dc.description.affiliationDepartment of Surgery
dc.description.affiliationLaboratory of Clinical Analysis
dc.description.affiliationDepartment of Microbiology Universidade do Oeste Paulista (UNOESTE)
dc.description.affiliationDepartment of Plastic Surgery
dc.description.affiliationDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP)
dc.format.extente25285
dc.identifierhttp://dx.doi.org/10.1097/MD.0000000000025285
dc.identifier.citationMedicine, v. 100, n. 13, p. e25285-, 2021.
dc.identifier.doi10.1097/MD.0000000000025285
dc.identifier.issn1536-5964
dc.identifier.scopus2-s2.0-85103745935
dc.identifier.urihttp://hdl.handle.net/11449/207569
dc.language.isoeng
dc.relation.ispartofMedicine
dc.sourceScopus
dc.titleDoes washing medical devices before and after use decrease bacterial contamination?: An in vitro studyen
dc.typeArtigo
unesp.author.orcid0000-0003-1674-7371 0000-0003-1674-7371 0000-0003-1674-7371 0000-0003-1674-7371[1]

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