Ovine β-globin gene: A new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection

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dc.contributor.authorOkino, Cintia Hiromi
dc.contributor.authorMéo Niciura, Simone Cristina
dc.contributor.authorBarbosa Toscano, João Henrique [UNESP]
dc.contributor.authorEsteves, Sérgio Novita
dc.contributor.authordos Santos, Isabella Barbosa [UNESP]
dc.contributor.authorvon Haehling, Marei Borsch [UNESP]
dc.contributor.authorFigueiredo, Amanda [UNESP]
dc.contributor.authorde Sena Oliveira, Márcia Cristina
dc.contributor.authorChagas, Ana Carolina de Souza
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:29:30Z
dc.date.available2021-06-25T10:29:30Z
dc.date.issued2021-06-01
dc.description.abstractTwo β-globin allelic haplotypes (A and B) were identified in domestic sheep, wherein animals which are homozygous for βB allele (BB haplotype) have a deletion of pre-adult βC-globin and consequently are less tolerant to anemia and hypoxia. Since Haemonchus contortus infection, is associated with severe anemia, studies performed from 1960s to 1990s investigated the association between β-globin haplotype and resistance against this parasite. However, the findings were controversial, pointing out from increased resistance in animals harboring the βA allele to inexistence of association. Thus, our study aimed to develop a qPCR for β-globin haplotype identification, and to evaluate the association between β-globin haplotype and resistance against H. contortus in a group of sheep submitted to artificial infection with this parasite. A total of 286 lambs of Morada Nova breed were experimentally challenged with 4000 H. contortus L3 and monitored for 112 days from weaning. Significantly improved (p < 0.05) phenotypic profiles (lower fecal egg counts, higher packed cell volume and birthweight) were observed for AA haplotype animals, especially when compared to BB animals, while AB animals were similar to BB. This is the first report of a qPCR assay for ovine β-globin haplotype identification. In view of significant differences of phenotypic profiles between haplotype groups, the developed qPCR may constitute an important tool for sheep producers to improve genetic selection of parasite resistant animals.en
dc.description.affiliationEmbrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234 s/n, Fazenda Canchim, PO Box 339
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipEmpresa Brasileira de Pesquisa Agropecuária
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 131537/2018-0
dc.description.sponsorshipIdEmpresa Brasileira de Pesquisa Agropecuária: 20.20.00.025.00.00
dc.description.sponsorshipIdFAPESP: 2017/00373-2
dc.description.sponsorshipIdFAPESP: 2018/16070-1
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2021.109434
dc.identifier.citationVeterinary Parasitology, v. 294.
dc.identifier.doi10.1016/j.vetpar.2021.109434
dc.identifier.issn1873-2550
dc.identifier.issn0304-4017
dc.identifier.scopus2-s2.0-85105102406
dc.identifier.urihttp://hdl.handle.net/11449/206278
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.sourceScopus
dc.subjectAllele
dc.subjectB haplotype
dc.subjectBeta globin C
dc.subjectMorada Nova
dc.subjectSheep
dc.titleOvine β-globin gene: A new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infectionen
dc.typeArtigo

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