MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)

dc.contributor.authorClivati, Debora
dc.contributor.authorGitzendanner, Matthew Aaron
dc.contributor.authorSilva Hilsdorf, Alexandre Wagner
dc.contributor.authorAraujo, Welington Luiz
dc.contributor.authorOliveira de Miranda, Vitor Fernandes [UNESP]
dc.contributor.institutionUniv Mogi das Cruzes
dc.contributor.institutionUniv Florida
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:30Z
dc.date.available2014-05-20T15:30:30Z
dc.date.issued2012-09-01
dc.description.abstractPremise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species.Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.en
dc.description.affiliationUniv Mogi das Cruzes, Nucleo Integrado Biotecnol, BR-08780911 São Paulo, Brazil
dc.description.affiliationUniv Florida, Dept Biol, Gainesville, FL 32611 USA
dc.description.affiliationUniv São Paulo, Dept Microbiol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 08/22239-9
dc.description.sponsorshipIdFAPESP: 07/58277-7
dc.description.sponsorshipIdCAPES: 5316/10-3
dc.format.extentE375-E378
dc.identifierhttp://dx.doi.org/10.3732/ajb.1200080
dc.identifier.citationAmerican Journal of Botany. St Louis: Botanical Soc Amer Inc, v. 99, n. 9, p. E375-E378, 2012.
dc.identifier.doi10.3732/ajb.1200080
dc.identifier.issn0002-9122
dc.identifier.urihttp://hdl.handle.net/11449/39854
dc.identifier.wosWOS:000308689800011
dc.language.isoeng
dc.publisherBotanical Soc Amer Inc
dc.relation.ispartofAmerican Journal of Botany
dc.relation.ispartofjcr2.788
dc.relation.ispartofsjr1,499
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAtlantic Foresten
dc.subjectbladderwortsen
dc.subjectendemic plantsen
dc.subjectgenetic diversityen
dc.subjectLentibulariaceaeen
dc.subjectUtricularia reniformisen
dc.titleMICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)en
dc.typeArtigo
dcterms.licensehttp://www.botany.org/ajb/AJBcopyright.pdf
dcterms.rightsHolderBotanical Soc Amer Inc

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