Short communication: Follicle superstimulation before ovum pick-up for in vitro embryo production in Holstein cows

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dc.contributor.authorOliveira, Louise H.
dc.contributor.authorSanches, Carlos P. [UNESP]
dc.contributor.authorSeddon, Adriano S.
dc.contributor.authorVeras, Marcio B.
dc.contributor.authorLima, Flavio A.
dc.contributor.authorMonteiro, Pedro L. J.
dc.contributor.authorWiltbank, Milo C.
dc.contributor.authorSartori, Roberto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAlcance Rural
dc.contributor.institutionCent Pioneiros
dc.contributor.institutionUniv Wisconsin
dc.date.accessioned2018-11-26T17:06:45Z
dc.date.available2018-11-26T17:06:45Z
dc.date.issued2016-11-01
dc.description.abstractThe objective was to evaluate in vitro embryo production (IVEP) in nonlactating Holstein cows after ovarian superstimulation. Cows were randomly assigned in a crossover design to 1 of 2 groups: control (n = 35), which was not synchronized and not treated with hormones before ovum pick-up (OPU), or hormone-treated (n = 35), in which wave emergence was synchronized and animals treated with porcine (p)-FSH in the presence of norgestomet before OPU. In the hormone-treated group, all follicles = 7 mm in diameter were aspirated for synchronization of wave emergence and cows received a norgestomet ear implant. After 36 h, treatment with p-FSH (6 doses of 40 mg each, 12 h apart, i.m.) started. Ovum pick-up from follicles >2 mm in diameter was performed 44 h after the last pFSH (coasting). Then, IVEP was performed. The total number of cumulus-oocyte complexes recovered (16.0 vs. 20.5 +/- 2.2) and number of grades I to III (viable) oocytes (10.7 vs. 12.3 +/- 1.6) did not differ between hormone-treated and control groups Additionally, no differences were found in the number of blastocysts per cow per OPU (3.0 vs. 2.6 +/- 0.5) or in blastocyst rates (17.1 vs. 12.2 +/- 2.4%) between hormone-treated and control, respectively. Thus, in this study, ovarian follicle superstimulation with p-FSH followed by coasting in nonlactating Holstein cows that had synchronization of wave emergence and progestin supplementation did not improve oocyte quality or IVEP compared to no hormonal treatment.en
dc.description.affiliationUniv Sao Paulo, Dept Anim Sci, BR-13418900 Piracicaba, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.affiliationAlcance Rural, BR-18400970 Itapeva, SP, Brazil
dc.description.affiliationCent Pioneiros, BR-84145000 Carambei, PR, Brazil
dc.description.affiliationUniv Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA
dc.description.affiliationUnespSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent9307-9312
dc.identifierhttp://dx.doi.org/10.3168/jds.2016-11306
dc.identifier.citationJournal Of Dairy Science. New York: Elsevier Science Inc, v. 99, n. 11, p. 9307-9312, 2016.
dc.identifier.doi10.3168/jds.2016-11306
dc.identifier.issn0022-0302
dc.identifier.urihttp://hdl.handle.net/11449/162071
dc.identifier.wosWOS:000385610700074
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Dairy Science
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbiotechnology
dc.subjectblastocyst
dc.subjectcattle
dc.subjectoocyte
dc.titleShort communication: Follicle superstimulation before ovum pick-up for in vitro embryo production in Holstein cowsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-6917-0057[8]

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