Effect of Atmospheric-Pressure Cold Plasma on Pathogenic Oral Biofilms and In Vitro Reconstituted Oral Epithelium

dc.contributor.authorDelben, Juliana Aparecida [UNESP]
dc.contributor.authorZago, Chaiene Evelin [UNESP]
dc.contributor.authorTyhovych, Natalia
dc.contributor.authorDuarte, Simone
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNYU
dc.date.accessioned2018-11-26T16:33:12Z
dc.date.available2018-11-26T16:33:12Z
dc.date.issued2016-05-25
dc.description.abstractConsidering the ability of atmospheric-pressure cold plasma (ACP) to disrupt the biofilm matrix and rupture cell structure, it can be an efficient tool against virulent oral biofilms. However, it is fundamental that ACP does not cause damage to oral tissue. So, this study evaluated (1) the antimicrobial effect of ACP on single-and dual-species biofilms of Candida albicans and Staphylococcus aureus as well as (2) the biological safety of ACP on in vitro reconstituted oral epithelium. Standardized cell suspensions of each microorganism were prepared for biofilm culture on acrylic resin discs at 37 degrees C for 48 hours. The biofilms were submitted to ACP treatment at 10 mm of plasma tip-to-sample distance during 60 seconds. Positive controls were penicillin G and fluconazole for S. aureus and C. albicans, respectively. The biofilms were analyzed through counting of viable colonies, confocal laser scanning microscopy, scanning electron microscopy and fluorescence microscopy for detection of reactive oxygen species. The in vitro reconstituted oral epithelium was submitted to similar ACP treatment and analyzed through histology, cytotoxocity test (LDH release), viability test (MTT assay) and imunnohistochemistry (Ki67 expression). All plasma-treated biofilms presented significant log10 CFU/mL reduction, alteration in microorganism/biofilm morphology, and reduced viability in comparison to negative and positive controls. In addition, fluorescence microscopy revealed presence of reactive oxygen species in all plasma-treated biofilms. Low cytotoxicity and high viability were observed in oral epithelium of negative control and plasma group. Histology showed neither sign of necrosis nor significant alteration in plasma-treated epithelium. Ki67-positive cells revealed maintenance of cell proliferation in plasma-treated epithelium. Atmospheric-pressure cold plasma is a promissing approach to eliminate single- nd dual-species biofilms of C. albicans and S. aureus without having toxic effects in oral epithelium.en
dc.description.affiliationSao Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo, Brazil
dc.description.affiliationNYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY USA
dc.description.affiliationUnespSao Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipGSK/IADR Innovation in Oral Care Award
dc.description.sponsorshipIdFAPESP: 2012/20699-6
dc.description.sponsorshipIdFAPESP: 2013/21388-7
dc.format.extent18
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0155427
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 11, n. 5, 18 p., 2016.
dc.identifier.doi10.1371/journal.pone.0155427
dc.identifier.fileWOS000376881700020.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/161554
dc.identifier.wosWOS:000376881700020
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleEffect of Atmospheric-Pressure Cold Plasma on Pathogenic Oral Biofilms and In Vitro Reconstituted Oral Epitheliumen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes3003130522427820[5]
unesp.author.orcid0000-0002-7375-4714[5]

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