Somatic cell count and type of intramammary infection impacts fertility from in vitro produced embryo transfer

Barbosa, L. F. S. P. [UNESP]; Oliveira, W. V. C. [UNESP]; Pereira, M. H. C. [UNESP]; Moreira, M. B.; Vasconcelos, C. G. C.; Silper, B. F.; Cerri, R. L. A.; Vasconcelos, J. L. M. [UNESP]

Somatic cell count and type of intramammary infection impacts fertility from in vitro produced embryo transfer

Arquivos

WOS000424732900037.pdf (348.19 KB)

Data

2018-03-01

Autores

Vasconcelos, C. G. C.

Silper, B. F.

Cerri, R. L. A.

Vasconcelos, J. L. M.

Editor

Elsevier B.V.

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

The objective of this study was to assess the impact of mastitis-causing bacteria and somatic cell count (SCC) on pregnancy per embryo transfer (P/ET) in Holstein-Gir crossbred (Girolando) lactating dairy cows. Cows (n = 1397) were subjected to a timed-embryo transfer protocol. Milk samples were collected two days before embryo transfer for SCC and bacteriological culture analyses. Pregnancy diagnosis was performed on days 31 and 66 after timed-embryo transfer. The animals were grouped according to the National Mastitis Council recommendations: Gram-positive environmental (EV+), Gram-negative environmental (EV-), Gram-positive contagious (C+), coagulase-negative staphylococci (CNS) and control (no bacterial growth). Additional analysis was made by categorizing bacteria based on degree of pathogenicity (Major or Minor). Bacterial growth reduced P/ET (P < .01) at both 31 and 66 days of gestation. The P/ET was lower (P < .05) at 31 days in EV- (30.1%) and EV+ (29.9%) groups and tended (P = .09) to be lower in the C+ group (36.6%) than the control group (44.0%). The P/ET from the Major group at 31 days of gestation was lower (P = .03) compared with the Minor and control groups (32.1 vs 41.1 vs 43.2%, respectively). Cows with SCC > 400,000 cells/mL had lower P/ET (P < .01) than animals with SCC < 200,000 cells/mL at both 31 (30.4% vs 40.8%) and 66 days (24.7% vs 32.2%) of gestation. Pregnancy loss was not different between bacterial isolates and SCC categories. Elevated SCC significantly reduced P/ET, whereas environmental agents and those with Major pathogenicity yielded the greatest reduction in NET. (C) 2017 Elsevier Inc. All rights reserved.