Disruption of giant unilamellar vesicles mimicking cell membranes induced by the pesticides glyphosate and picloram

dc.contributor.authorLemma, Tibebe [UNESP]
dc.contributor.authorRuiz, Gilia Cristine Marques [UNESP]
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.authorConstantino, Carlos J.L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T17:08:20Z
dc.date.available2019-10-06T17:08:20Z
dc.date.issued2019-07-01
dc.description.abstractGiant unilamellar vesicles (GUVs) have been one of the most extensively investigated membrane model to study cell membrane-ligand interactions. In this study, we investigated the interaction between glyphosate and picloram with GUVs made with sphingomyelin (SM), cholesterol (CHOL), and dioleoyl-sn-glycerol-3-phosphocholine (DOPC) (DOPC/SM (1:1), DOPC/CHOL/SM (1:1:1)) in a physiological environment using confocal and phase contrast microscopy. At high pesticide concentrations (70 to 90 μM), we generally found the GUVs undergoing a physical such as contouring, elongation, and eventually lose their characteristic spherical shape. In addition, to determine the comparative effect of the pesticides, control experiments were performed using GUVs made with only DOPC and DOPC/SM 1:1. The results show that, at low concentration (0.5 μM), a significant effect was observed during a 30 min incubation time. These findings also suggest that cholesterol may play a significant role in the permeability of the vesicle against the action of the pesticides, which have important biological implications on the lipid composition of the membrane.en
dc.description.affiliationFaculdade de Ciências e Tecnologia (FCT)-Universidade Estadual Paulista (UNESP)-Presidente Prudente
dc.description.affiliationSão Carlos Institute of Physics, University of Sao Paulo, CP 369
dc.description.affiliationUnespFaculdade de Ciências e Tecnologia (FCT)-Universidade Estadual Paulista (UNESP)-Presidente Prudente
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/14262-7
dc.description.sponsorshipIdFAPESP: 2016/06424-5
dc.identifierhttp://dx.doi.org/10.1016/j.bpc.2019.106176
dc.identifier.citationBiophysical Chemistry, v. 250.
dc.identifier.doi10.1016/j.bpc.2019.106176
dc.identifier.issn1873-4200
dc.identifier.issn0301-4622
dc.identifier.scopus2-s2.0-85064955415
dc.identifier.urihttp://hdl.handle.net/11449/190289
dc.language.isoeng
dc.relation.ispartofBiophysical Chemistry
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectConfocal microscopy
dc.subjectGlyphosate, picloram
dc.subjectGUVs
dc.subjectLipids
dc.subjectPhase-contrast
dc.titleDisruption of giant unilamellar vesicles mimicking cell membranes induced by the pesticides glyphosate and picloramen
dc.typeArtigo
unesp.author.lattes6118325967319836[4]
unesp.author.orcid0000-0002-5921-3161[4]

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