Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro

dc.contributor.authorRoncari Rocha, Guilherme [UNESP]
dc.contributor.authorSims, Kenneth R.
dc.contributor.authorXiao, Baixue
dc.contributor.authorKlein, Marlise I. [UNESP]
dc.contributor.authorBenoit, Danielle S.W.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Rochester
dc.description.abstractBackground: Dental caries is a multifactorial disease caused by pathogenic biofilm. In particular, Streptococcus mutans synthesizes biofilm exopolysaccharides, while Candida albicans is associated with the development of severe carious lesions. Aim: This study aimed to prevent the formation of S. mutans and C. albicans biofilms by exploiting pH-sensitive nanoparticle carriers (NPCs) with high affinity to exopolysaccharides to increase the substantivity of multi-targeted antibiofilm drugs introduced topically in vitro. Methods: Dual-species biofilms were grown on saliva-coated hydroxyapatite discs with sucrose. Twice-daily, 1.5 min topical treatment regimens of unloaded and drug-loaded NPC were used. Drugs included combinations of two or three compounds with distinct, complementary antibiofilm targets: tt-farnesol (terpenoid; bacterial acid tolerance, fungal quorum sensing), myricetin (flavonoid; exopolysaccharides inhibitor), and 1771 (lipoteichoic acid inhibitor; bacterial adhesion and co-aggregation). Biofilms were evaluated for biomass, microbial population, and architecture. Results: NPC delivering tt-farnesol and 1771 with or without myricetin completely prevented biofilm formation by impeding biomass accumulation, bacterial and fungal population growth, and exopolysaccharide matrix deposition (vs. control unloaded NPC). Both formulations hindered acid production, maintaining the pH of spent media above the threshold for enamel demineralization. However, treatments had no effect on pre-established dual-species biofilms. Conclusion: Complementary antibiofilm drug-NPC treatments prevented biofilm formation by targeting critical virulence factors of acidogenicity and exopolysaccharides synthesis.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics São Paulo State University
dc.description.affiliationDepartment of Biomedical Engineering University of Rochester
dc.description.affiliationMaterials Science Program University of Rochester
dc.description.affiliationDepartment of Orthopaedics and Center for Musculoskeletal Research University of Rochester
dc.description.affiliationCenter for Oral Biology University of Rochester
dc.description.affiliationDepartment of Chemical Engineering University of Rochester
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics São Paulo State University
dc.identifier.citationJournal of Oral Microbiology, v. 14, n. 1, 2022.
dc.relation.ispartofJournal of Oral Microbiology
dc.subjectCandida albicans
dc.subjectdental caries
dc.subjectStreptococcus mutans
dc.titleNanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitroen