Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels

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dc.contributor.authorBraga, Gabriela Pacheco de Almeida [UNESP]
dc.contributor.authorCaiaffa, Karina Sampaio [UNESP]
dc.contributor.authorRabelo, Rafaela Laruzo [UNESP]
dc.contributor.authorSantos, Vanessa Rodrigues dos [UNESP]
dc.contributor.authorSouza, Amanda Caselato Andolfatto [UNESP]
dc.contributor.authorRibeiro, Lucas da Silva
dc.contributor.authorCamargo, Emerson Rodrigues de
dc.contributor.authorPrakki, Anuradha
dc.contributor.authorDuque, Cristiane [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversity of Toronto
dc.date.accessioned2023-07-29T13:48:15Z
dc.date.available2023-07-29T13:48:15Z
dc.date.issued2023-03-01
dc.description.abstractThis study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules’ deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.en
dc.description.affiliationDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos (UFSCar), SP
dc.description.affiliationDental Research Institute Faculty of Dentistry University of Toronto
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/jfb14030139
dc.identifier.citationJournal of Functional Biomaterials, v. 14, n. 3, 2023.
dc.identifier.doi10.3390/jfb14030139
dc.identifier.issn2079-4983
dc.identifier.scopus2-s2.0-85151120660
dc.identifier.urihttp://hdl.handle.net/11449/248591
dc.language.isoeng
dc.relation.ispartofJournal of Functional Biomaterials
dc.sourceScopus
dc.subjectalkaline phosphatase
dc.subjectcytotoxicity
dc.subjectdentin mineralization
dc.subjectflavonoids
dc.subjecthydrogel
dc.subjectodontoblasts
dc.titleCytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogelsen
dc.typeArtigo
unesp.author.orcid0000-0001-8725-2136[4]
unesp.author.orcid0000-0002-8969-9043[8]
unesp.author.orcid0000-0002-2575-279X[9]

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Artigos - Odontologia Restauradora - FOA