Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study

dc.contributor.authorDe Paula Ramos, Lucas [UNESP]
dc.contributor.authorDa Rocha Santos, Carlos Eduardo [UNESP]
dc.contributor.authorCamargo Reis Mello, Daphne [UNESP]
dc.contributor.authorNishiama Theodoro, Lígia [UNESP]
dc.contributor.authorDe Oliveira, Felipe Eduardo [UNESP]
dc.contributor.authorBack Brito, Graziella N. [UNESP]
dc.contributor.authorCampos Junqueira, Juliana [UNESP]
dc.contributor.authorCardoso Jorge, Antonio Olavo [UNESP]
dc.contributor.authorDias De Oliveira, Luciane [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:08:24Z
dc.date.available2018-12-11T17:08:24Z
dc.date.issued2016-01-01
dc.description.abstractThis study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p<0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, Francisco José Longo 777
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, Francisco José Longo 777
dc.identifierhttp://dx.doi.org/10.1155/2016/3521413
dc.identifier.citationScientific World Journal, v. 2016.
dc.identifier.doi10.1155/2016/3521413
dc.identifier.file2-s2.0-85006237860.pdf
dc.identifier.issn1537-744X
dc.identifier.issn2356-6140
dc.identifier.scopus2-s2.0-85006237860
dc.identifier.urihttp://hdl.handle.net/11449/173934
dc.language.isoeng
dc.relation.ispartofScientific World Journal
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleKlebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro studyen
dc.typeArtigo
unesp.author.lattes0053567153623569[8]
unesp.author.orcid0000-0002-1747-6158[8]

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