Optical diagnosis of actinic cheilitis by infrared spectroscopy

dc.contributor.authordas Chagas e Silva de Carvalho, Luis Felipe
dc.contributor.authorPereira, Thiago Martini
dc.contributor.authorMagrini, Taciana Depra
dc.contributor.authorCavalcante, Ana Sueli Rodrigues [UNESP]
dc.contributor.authorda Silva Martinho, Herculano
dc.contributor.authorAlmeida, Janete Dias [UNESP]
dc.contributor.institutionUniversidade do Vale do Paraíba
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2018-12-11T17:29:37Z
dc.date.available2018-12-11T17:29:37Z
dc.date.issued2016-12-01
dc.description.abstractActinic cheilitis (AC) is considered a potentially malignant disorder of the lip. Biomolecular markers study is important to understand malignant transformation into squamous cell carcinoma. Fourier transform infra red (FT-IR) spectroscopy was used to analyze AC in this study. Objectives The aim of the study was to evaluate if FT-IR spectral regions of nucleic acids and collagen can help in early diagnosis of malignant transformation. Methods Tissues biopsies of 14 patients diagnosed with AC and 14 normal tissues were obtained. FT-IR spectra were measured at five different points resulting in 70 spectra of each. Analysis of Principal components analysis (PCA) and linear discrimination analysis (LDA) model were also used. In order to verify the statistical difference in the spectra, Mann-Whitney U test was performed in each variable (wavenumber) with p-value <0.05. Results After the Mann-Whitney U test the vibrational modes of CO (Collagen 1), PO2 (Nucleic Acids) and C[dbnd]O asymmetric (Triglycerides/Lipids) were observed as a possible spectral biomarker. These bands were chosen because they represent the vibrational modes related to collagen and DNA, which are supposed to be changed in AC samples. Based on the PCA-LDA results, the predictive model corresponding to the area under the curve was 0.91 for the fingerprint region and 0.83 for the high wavenumber region, showing the greater accuracy of the test. Conclusions FT-IR changes in collagen and nucleic acids could be used as molecular biomarkers for malignant transformation.en
dc.description.affiliationLaboratory of Biomedical Vibrational Spectroscopy Universidade do Vale do Paraíba
dc.description.affiliationInstituto de Ciência e Tecnologia Universidade Federal de São Paulo
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology Univ Estadual Paulista (UNESP)
dc.description.affiliationCentro de Ciências Naturais e Humanas Universidade Federal do ABC
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology Univ Estadual Paulista (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent27-34
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2016.07.013
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 16, p. 27-34.
dc.identifier.doi10.1016/j.pdpdt.2016.07.013
dc.identifier.file2-s2.0-84988432332.pdf
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-84988432332
dc.identifier.urihttp://hdl.handle.net/11449/178285
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.relation.ispartofsjr0,647
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectActinic cheilitis
dc.subjectFT-IR spectroscopy
dc.subjectOptical biopsy
dc.subjectOral cancer
dc.titleOptical diagnosis of actinic cheilitis by infrared spectroscopyen
dc.typeArtigo
unesp.author.lattes8987735916695328[4]
unesp.author.orcid0000-0001-7078-7708[4]

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