Genetic relationships among Arachis species based on AFLP

dc.contributor.authorGimenes, Marcos A. [UNESP]
dc.contributor.authorLopes, Catalina R. [UNESP]
dc.contributor.authorValls, Jose F.M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2014-05-20T13:50:14Z
dc.date.available2014-05-20T13:50:14Z
dc.date.issued2002-01-01
dc.description.abstractAmplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI - ACC / Mse I - CTG, while the lowest was 108, with Eco RI - ACT / Mse I - CTT. In general, the genetic relationships established using AFLPs agreed with the classification established using morphology and crossability data. The results indicated that AFLPs are good markers for establishing the relationships among Arachis species. The polymorphism detected in A. hypogaea by this method was higher than the one found with other markers, like RAPDs and RFLPs. However, our data suggest that the polymorphism detected be using AFLP with only three primer combinations is still too low to be used for any kind of genetic study in this species.en
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Departamento de Genética
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Recursos Genéticos e Biotecnologia (CENARGEN)
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Departamento de Genética
dc.format.extent349-353
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572002000300017
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 3, p. 349-353, 2002.
dc.identifier.doi10.1590/S1415-47572002000300017
dc.identifier.fileS1415-47572002000300017.pdf
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572002000300017
dc.identifier.urihttp://hdl.handle.net/11449/17937
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectAFLPen
dc.subjectArachisen
dc.subjectpeanuten
dc.subjecttaxonomic relationshipen
dc.titleGenetic relationships among Arachis species based on AFLPen
dc.typeArtigo

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
S1415-47572002000300017.pdf
Tamanho:
98.5 KB
Formato:
Adobe Portable Document Format

Licença do Pacote

Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções