Impact of Seasonal and Organ-Related Fluctuations on the Anthelmintic Properties and Chemical Profile of Cladium mariscus (L.) Pohl Extracts

dc.contributor.authorOliveira, Marta
dc.contributor.authorLima, Caroline Sprengel [UNESP]
dc.contributor.authorLlorent-Martínez, Eulogio J.
dc.contributor.authorHoste, Hervé
dc.contributor.authorCustódio, Luísa
dc.contributor.institutionUniversity of Algarve
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Jaén
dc.contributor.institutionENVT
dc.date.accessioned2023-03-01T20:17:05Z
dc.date.available2023-03-01T20:17:05Z
dc.date.issued2022-06-23
dc.description.abstractThe use of plants and their metabolites stands as a promising option to tackle parasitic infections by gastrointestinal nematodes (GIN) in integrated control strategies. Still, the influence of environmental and phenological factors, and their interactions, in the wild on the metabolomics and biological properties of target plant species, is often disregarded. In this work, we hypothesized that variations in the anthelmintic (AH) properties and chemical composition of extracts from the salt tolerant species Cladium mariscus L. Pohl (sawgrass) may be influenced by seasonal factors and organ-parts. To test this hypothesis, acetone/water extracts were prepared from dried biomass obtained from aerial organs collected from sawgrass in consecutive seasons and tested against Haemonchus contortus and Trichostrongylus colubriformis by the larval exsheathment inhibition assay (LEIA) and egg hatching inhibition assay (EHIA). To ascertain the role of plant organ, the activity of leaves and inflorescences extracts from summer samples was compared. The role of polyphenols in the anthelmintic activity depending on GINs and fluctuations across seasons and plant organs was assessed using polyvinylpolypyrrolidone (PVPP), coupled with an in-depth chemical profiling analysis using high-performance liquid chromatography completed with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). Main differences in anthelmintic activities were observed for summer and autumn samples, for both assays. Moreover, inflorescences’ extracts were significantly more active than those from leaves against both parasite species on EHIA and against H. contortus on LEIA. Application of PVPP totally inhibit the AH effects based on EHIA and only partly for LEIA. Non-treated PVPP extracts were predominantly composed of flavan-3-ols, proanthocyanidins, luteolin and glycosylated flavonoids, while two flavonoid glycosides were quantified in all PVPP-treated samples. Thus, the activity of such compounds should be further explored, although some unknown metabolites remain to be identified. This study reinforces the hypothesis of the AH potential of sawgrass and of its polyphenolic metabolites uses as nutraceutical and/or phytotherapeutic drugs.en
dc.description.affiliationCentre of Marine Sciences University of Algarve
dc.description.affiliationLaboratory of Antibiotics and Chemotherapeutics Instituto de Biociências Letras e Ciências Exatas (IBILCE) São Paulo State University
dc.description.affiliationDepartment of Physical and Analytical Chemistry Faculty of Experimental Sciences University of Jaén
dc.description.affiliationIHAP Université de Toulouse INRAE ENVT
dc.description.affiliationUniversité de Toulouse ENVT
dc.description.affiliationUnespLaboratory of Antibiotics and Chemotherapeutics Instituto de Biociências Letras e Ciências Exatas (IBILCE) São Paulo State University
dc.identifierhttp://dx.doi.org/10.3389/fpls.2022.934644
dc.identifier.citationFrontiers in Plant Science, v. 13.
dc.identifier.doi10.3389/fpls.2022.934644
dc.identifier.issn1664-462X
dc.identifier.scopus2-s2.0-85133838811
dc.identifier.urihttp://hdl.handle.net/11449/240437
dc.language.isoeng
dc.relation.ispartofFrontiers in Plant Science
dc.sourceScopus
dc.subjectanthelmintic
dc.subjectgastrointestinal nematodes
dc.subjecthalophytes
dc.subjectpolyphenols
dc.subjectsalt tolerant plants
dc.subjectsmall ruminants
dc.titleImpact of Seasonal and Organ-Related Fluctuations on the Anthelmintic Properties and Chemical Profile of Cladium mariscus (L.) Pohl Extractsen
dc.typeArtigo

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