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Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp citri during infection of Citrus sinensis

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dc.contributor.authorJacob, Tiago R. [UNESP]
dc.contributor.authorLaia, Marcelo L. [UNESP]
dc.contributor.authorFerro, Jesus A. [UNESP]
dc.contributor.authorFerro, Maria I. T. [UNESP]
dc.contributor.institutionUniv Fed Vales Jequitinhonha & Mucuri
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:17:41Z
dc.date.available2014-05-20T13:17:41Z
dc.date.issued2011-06-01
dc.description.abstractXanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.en
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, BR-39100000 Diamantina, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundo de Defesa da Citricultura (Fundecitrus)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1177-1184
dc.identifierhttp://dx.doi.org/10.1007/s10529-011-0552-5
dc.identifier.citationBiotechnology Letters. Dordrecht: Springer, v. 33, n. 6, p. 1177-1184, 2011.
dc.identifier.doi10.1007/s10529-011-0552-5
dc.identifier.issn0141-5492
dc.identifier.urihttp://hdl.handle.net/11449/4063
dc.identifier.wosWOS:000291655200015
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBiotechnology Letters
dc.relation.ispartofjcr1.846
dc.relation.ispartofsjr0,621
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGene expressionen
dc.subjectqRT-PCRen
dc.subjectReference genesen
dc.subjectXanthomonas citri subsp. citrien
dc.titleSelection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp citri during infection of Citrus sinensisen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0001-6366-4558[2]
unesp.author.orcid0000-0002-3966-1303[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentTecnologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias