A standard protocol for obtaining fish chromosomes under post-mortem conditions

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de Cascia Barreto Netto, Maria Rita
Pauls, Erica
Affonso, Paulo Roberto A. de Mello

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Elsevier B.V.


In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed. (c) 2006 Elsevier Ltd. All rights reserved.



chromosomes, freshwater fish, marine fish, in vitro cytogenetical technique

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Micron. Oxford: Pergamon-Elsevier B.V., v. 38, n. 3, p. 214-217, 2007.