Comparative spermatozoal ultrastructure and molecular analysis in dromiid crabs and their phylogenetic implications for Dromiidae and Podotremata (Decapoda: Brachyura)

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dc.contributor.authorGarcia Bento, Maria Alice [UNESP]
dc.contributor.authorMiranda, Ivana [UNESP]
dc.contributor.authorMantelatto, Fernando Luis
dc.contributor.authorZara, Fernando José [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:03:38Z
dc.date.available2019-10-06T16:03:38Z
dc.date.issued2018-11-01
dc.description.abstractWe described the spermatozoal ultrastructure and conducted a molecular analysis of Dromiidae Hypoconcha parasitica, Hypoconcha arcuata, Moreiradromia antillensis and Dromia erythropus. To elucidate the relationship between the different species of this brachyuran group, we also compared the spermatozoal morphologies and phylogenetic positioning among species of Dromiidae, Dromioidea and Podotremata. Specimens were collected from the northern coast of São Paulo, Brazil and were fixed and processed followed by transmission electron microscopy and molecular analysis routines. The Dromiidae spermatozoa studied are characterized by a discoidal acrosome, with three or four concentric zones, which are centrally separated by a bilaterally capitate perforatorial chamber, with a “mushroom”-shaped apex in the Hypoconchinae and a “T-shape” in Dromiinae. Above the perforatorial chamber, there is an apical protuberance, continuous with the subopercular region and the operculum, which forms a low, centrally perforated dome. Under differential interference contrast microscopy, the spermatozoa show 3 to 4 radial arms. The spermatozoal characters in Hypoconchinae and Dromiinae do not separate these subfamilies from the Dromiidae and Dromioidea. Ultrastructural differentiation was only found between representative Dromioidea and other Podotremata. Thus, the spermiotaxonomy of these Hypoconcha, Moreiradromia and Dromia species corroborated previous morphological and molecular studies, supporting the monophyly of Dromiidae and Dynomenidae in relation to Homolidae and Latreilliidae.en
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) FCAV Departamento de Biologia Aplicada à Agropecuária Laboratório de Morfologia de Invertebrados (IML) e Centro de Aquicultura da Unesp (CAUNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Jaboticabal
dc.description.affiliationLaboratório de Bioecologia e Sistemática de Crustáceos (LBSC) Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto (FFCLRP) Universidade de São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto
dc.description.affiliationUniversidade Estadual Paulista (UNESP) IB, Avenida 24 A, 1515
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) FCAV Departamento de Biologia Aplicada à Agropecuária Laboratório de Morfologia de Invertebrados (IML) e Centro de Aquicultura da Unesp (CAUNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Jaboticabal
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) IB, Avenida 24 A, 1515
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/50188-8
dc.description.sponsorshipIdFAPESP: 2014/21294-5
dc.description.sponsorshipIdFAPESP: 2016/10294-4
dc.format.extent627-642
dc.identifierhttp://dx.doi.org/10.1016/j.asd.2018.10.001
dc.identifier.citationArthropod Structure and Development, v. 47, n. 6, p. 627-642, 2018.
dc.identifier.doi10.1016/j.asd.2018.10.001
dc.identifier.issn1873-5495
dc.identifier.issn1467-8039
dc.identifier.scopus2-s2.0-85055737542
dc.identifier.urihttp://hdl.handle.net/11449/188300
dc.language.isoeng
dc.relation.ispartofArthropod Structure and Development
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDromiid
dc.subjectPhylogeny
dc.subjectPodotremata
dc.subjectPrimitive crabs
dc.subjectTransmission electron microscopy
dc.titleComparative spermatozoal ultrastructure and molecular analysis in dromiid crabs and their phylogenetic implications for Dromiidae and Podotremata (Decapoda: Brachyura)en
dc.typeArtigo
unesp.author.orcid0000-0002-3552-2189[1]
unesp.author.orcid0000-0002-8820-6681[2]
unesp.author.orcid0000-0002-8497-187X[3]
unesp.author.orcid0000-0002-7664-7674 0000-0002-7664-7674[4]
unesp.departmentBiologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias