Propranolol inhibits cell viability and expression of the pro-tumorigenic proteins Akt, NF-ĸB, and VEGF in oral squamous cell carcinoma

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dc.contributor.authorShibuya, Caroline Miho [UNESP]
dc.contributor.authorTjioe, Kellen Cristine [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha [UNESP]
dc.contributor.authorBernabé, Daniel Galera [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:40:14Z
dc.date.available2022-04-29T08:40:14Z
dc.date.issued2022-04-01
dc.description.abstractBackground: Propranolol (PPL) has been suggested as an option for the treatment of various types of cancer. However, data regarding its effectiveness against oral cancer are scarce. Thus, we aimed to evaluate the antitumor potential of PPL in oral squamous cell carcinoma (OSCC) in vitro. Methods: OSCC cell lines, SCC-9, SCC-25, and Cal27, were treated with PPL at different times and concentrations. OSCC cells were treated with PPL alone or in combination with cisplatin (CDDP) or 5-fluorouracil (5-FU). Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of phosphorylated (p)-Akt, p-S6, p-PTEN, p-P65, and VEGF was verified by immunofluorescence. The migratory activity of OSCC cells was evaluated using a wound-healing assay. Results: PPL reduced OSCC cell viability in a dose- and time-dependent manner. Concentrations above 300 µM, 110 µM, and 100 µM for SCC-9, Cal27, and SCC-25, respectively, significantly eliminated tumor cells. The combination of PPL with CDDP and 5-FU enhanced their antitumor effects. There was a modest difference between the use of the IC30 and IC50 of PPL in the combinatory options. PPL downregulated p-P65 NF-ĸB and VEGF expression in SCC-9 and Cal27 cells but not in SCC-25 cells. PPL inhibited the phosphorylation of Akt and s6 and increased the phosphorylation of PTEN in all OSCC cell lines studied. PPL inhibited OSCC cell migration after 24 h of treatment. Conclusion: PPL was effective against oral cancer cells and enhanced standard-of-care. PPL inhibited cell viability and the expression of pAkt, NF-ĸB, and VEGF.en
dc.description.affiliationLaboratory of Immunopharmacology Department of Basic Sciences School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationPsychoneuroimmunology Laboratory Psychosomatic Research Center Oral Oncology Center School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespLaboratory of Immunopharmacology Department of Basic Sciences School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespPsychoneuroimmunology Laboratory Psychosomatic Research Center Oral Oncology Center School of Dentistry São Paulo State University (Unesp), São Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: # 2016/03965-2
dc.description.sponsorshipIdFAPESP: # 2017/18650-2
dc.description.sponsorshipIdFAPESP: #2016/00051-2
dc.description.sponsorshipIdFAPESP: #2016/24685-3
dc.description.sponsorshipIdFAPESP: #2016/25255-0
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2022.105383
dc.identifier.citationArchives of Oral Biology, v. 136.
dc.identifier.doi10.1016/j.archoralbio.2022.105383
dc.identifier.issn1879-1506
dc.identifier.issn0003-9969
dc.identifier.scopus2-s2.0-85125493670
dc.identifier.urihttp://hdl.handle.net/11449/230479
dc.language.isoeng
dc.relation.ispartofArchives of Oral Biology
dc.sourceScopus
dc.subjectbeta-adrenergic receptor
dc.subjectBeta-blockers
dc.subjectDistress
dc.subjecthead and neck cancer
dc.subjectHead and neck squamous cell carcinoma
dc.subjectNF-kB
dc.subjectNorepinephrine
dc.subjectoral cancer
dc.subjectoral neoplasms
dc.subjectoral squamous cell carcinoma
dc.subjectPropranolol
dc.titlePropranolol inhibits cell viability and expression of the pro-tumorigenic proteins Akt, NF-ĸB, and VEGF in oral squamous cell carcinomaen
dc.typeArtigo

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