Technical modification of indirect immunofluorescent antibody test using filter paper blood eluates

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dc.contributor.authorDe Franco, Marcello F.
dc.contributor.authorChamma, L. G.
dc.date.accessioned2023-07-29T13:58:48Z
dc.date.available2023-07-29T13:58:48Z
dc.date.issued1973-01-01
dc.description.abstractAn indirect immunofluorescence test using small discs (0.4 cm in diameter) cut from blood smears on filter paper is described. Discs plus three drops of phosphate-buffered saline are laid on antigenic areas on slides, which are then incubated. During incubation, eluted antibodies react with antigen. The method was tested with 10 eluates from healthy persons and with 30 eluates from three groups of 10 patients with Chagas’ disease, schistosomiasis Mansoni and South American blastomycosis, respectively. Sera from the same blood samples were tested also by conventional indirect immunofluorescence tests. Both tests gave similar results. The method proposed is useful as a rapid qualitative screening test when dealing with a large number of samples. © 1973 S. Karger AG, Basel.en
dc.description.affiliationDepartment of Pathology, Faculdade de Ciências Médicas e Biológicas de Botucatu, São Paulo
dc.format.extent692-696
dc.identifierhttp://dx.doi.org/10.1159/000230971
dc.identifier.citationInternational Archives of Allergy and Immunology, v. 44, n. 5, p. 692-696, 1973.
dc.identifier.doi10.1159/000230971
dc.identifier.issn1423-0097
dc.identifier.issn1018-2438
dc.identifier.scopus2-s2.0-0015832939
dc.identifier.urihttp://hdl.handle.net/11449/248969
dc.language.isoeng
dc.relation.ispartofInternational Archives of Allergy and Immunology
dc.sourceScopus
dc.titleTechnical modification of indirect immunofluorescent antibody test using filter paper blood eluatesen
dc.typeArtigo

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