The role of glucose metabolism in cardiac remodeling induced by tobacco smoke exposure

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dc.contributor.authorAzevedo, Paula S. [UNESP]
dc.contributor.authorFerreira, Vanessa C. M. P. [UNESP]
dc.contributor.authorSantos, Priscila [UNESP]
dc.contributor.authorGonçalves, Andréa de Freitas [UNESP]
dc.contributor.authorOkoshi, Marina P. [UNESP]
dc.contributor.authorFernandes, Ana Angelica Henrique [UNESP]
dc.contributor.authorRoscani, Meliza Goi [UNESP]
dc.contributor.authorPolegato, Bertha F. [UNESP]
dc.contributor.authorPaiva, Sergio [UNESP]
dc.contributor.authorZornoff, Leonardo A. M. [UNESP]
dc.contributor.authorMinicucci, M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-07-07T12:37:24Z
dc.date.available2016-07-07T12:37:24Z
dc.date.issued2015
dc.description.abstractTherefore The aim of this study is to investigate the cardiac glucose metabolism pathways in cardiac remodeling induced by TS. Methods 20 animals were allocated into 2 groups: group of animals exposed to tobacco smoke (TS) and group animals not exposed to cigarette smoke (C). After 2 months the animals were subjected to: a) echocardiographic study; e) spectrophotometric evaluation of energy metabolism enzymes and f) Western blot for Akt and AMPK.The TS group showed larger left atrium size and lower ejection fraction than those of group. The animals showed the same body weight (p = 0.3), heart rate ( p = 0.4) and mean arterial pressure. As regards the analysis of energy metabolism enzymes, phosphofructokinase (PFK) (C 138 ± 24, TS=198 ± 31, p = 0.003), LDH (C =121 ± 31, TS= 167 ± 27, p = 0.017) and pyruvate dehydrogenase (PDH) (C=208 ± 18, TS= 211 ± 27, p=0,83) were greater in the TS group and citrate synthase (C =39,0 ±2.32, 25.53 ± TS=5.36, p <0.001), complex I (C =5.03 ± 0.48, TS=2.75 ± 0.62, p <0.001), complex II (C=5.32 ± 0.93; TS= 2.48 ± 0.78, p <0.001) , ATP synthase (C=57.91 ± 7.24, TS= 21.18 ± 4.84 p <0.001) were lower in the TS group. Akt/Aktp was similar between the groups. AMPK was reduced in CS group (p<0,001).Conclusion: During early cardiac remodeling, there is an increase in glucose metabolism. In this study LDH and PFK , that represents glycolytic pathways were increased. However, the citric acid cicle and mitochondrial respiratory chain may be compromised. One of the key modulators of these features may be AMPK.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina (FMB) - Botucatu
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Bioquímica, Instituto de Biociências (IBB), Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina (FMB) - Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Bioquímica, Instituto de Biociências (IBB), Botucatu
dc.format.extent370
dc.identifierhttp://www.fasebj.org/content/29/1_Supplement/LB370.short?related-urls=yes&legid=fasebj;29/1_Supplement/LB370
dc.identifier.citationThe FASEB Journal, v. 29, p. 370, 2015.
dc.identifier.issn0892-6638
dc.identifier.urihttp://hdl.handle.net/11449/141225
dc.language.isoeng
dc.relation.ispartofThe FASEB Journal
dc.relation.ispartofjcr5.595
dc.relation.ispartofsjr2,438
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.titleThe role of glucose metabolism in cardiac remodeling induced by tobacco smoke exposureen
dc.typeArtigo
unesp.author.lattes4563764623232492[8]
unesp.author.orcid0000-0002-2875-9532[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentQuímica e Bioquímicapt

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