Microscopic, morphometric and ultrastructural analysis of anastomotic healing in the intestine of normal and diabetic rats
Nenhuma Miniatura disponível
Data
2008-04-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
The purpose of this study was to investigate if experimental alloxanic diabetes could cause qualitative changes in intestinal anastomoses of the terminal ileum and distal colon in rats, as compared to controls. 192 male Wistar rats, weighing ± 300g were split into four experimental groups of 48 animals each, after 3 months of follow-up: a control group with ileum anastomoses (G1), a control group with colon anastomoses (G2), a diabetic group with ileum anastomoses (G3) and a diabetic group with colon anastomoses (G4). Animals were evaluated and sacrificed on days 4, 14, 21 and 30 after surgery, and fragments of the small and large intestine where the anastomoses were performed were removed. Samples from 6 animals from each sacrifice moment were submitted to ultrastructural analysis of the collagen fibers using a scanning electron microscope and samples from another 6 animals were submitted to histopathology and optical microscopy studies using picrosirius red-staining. Histopathological analysis of picrosirius red-stained anastomosis slides using an optical microscope at 40x magnification showed that the distribution of collagen fibers was disarranged and also revealed a delay in scar tissue retraction. The morphometric study revealed differences in the collagen filled area for the ileum anastomoses 14 days post surgery whereas, in the case of colon anastomoses, differences were observed at days 4 and 30 post surgery, with higher values in the diabetic animals. Ultrastructure analysis of the ileum and colon anastomoses using a scanning electron microscope revealed fewer wide collagen fibers, the presence of narrower fibers and a disarranged distribution of the collagen fibers. We conclude that diabetes caused qualitative changes in scar tissue as well as in the structural arrangement of collagen fibers, what could explain the reduced wound strength in the anastomosis of diabetic animals. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart.
Descrição
Palavras-chave
Experimental diabetes, Healing, Intestinal anastomosis, collagen fiber, alloxan diabetes mellitus, animal experiment, animal model, animal tissue, controlled study, descending colon, histopathology, ileum, intestine anastomosis, microscopy, morphometrics, nonhuman, priority journal, rat, scanning electron microscopy, scar, tissue structure, ultrastructure analysis and electron microscopy, Wistar rat, wound healing, Anastomosis, Surgical, Animals, Diabetes Mellitus, Experimental, Intestines, Male, Microscopy, Electron, Rats, Rats, Wistar, Reference Values, Wound Healing
Idioma
Inglês
Como citar
Experimental and Clinical Endocrinology and Diabetes, v. 116, n. 4, p. 198-202, 2008.