Molecular detection of Hepatozoon spp. in non-hematophagous bats in Brazil

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dc.contributor.authorPerles, Livia [UNESP]
dc.contributor.authorIkeda, Priscila [UNESP]
dc.contributor.authorFrancisco, Gabriela de Vasconcellos [UNESP]
dc.contributor.authorTorres, Jaire Marinho [UNESP]
dc.contributor.authorOliveira, Carina Elisei de
dc.contributor.authorLourenco, Elizabete Captivo
dc.contributor.authorHerrera, Heitor Miraglia
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorAndre, Marcos Rogerio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Catolica Dom Bosco
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.date.accessioned2020-12-10T19:54:40Z
dc.date.available2020-12-10T19:54:40Z
dc.date.issued2020-05-01
dc.description.abstractEven though Hepatozoon spp. has been molecularly detected in several wild animals in Brazil, there is no report on the occurrence of Hepatozoon spp. DNA in bats in Brazil. This study aimed at detecting Hepatozoon, in addition to ectoparasites, in non-hematophagous bats sampled in central-western Brazil using blood smears, hematoxylineosin (HE)-staining liver/spleen preparations and molecular and phylogenetic techniques. A total of 135 spleen, 127 liver, and 133 blood samples were collected from 135 non-hematophagous bats from 12 different species in two different sites in Campo Grande, Mato Grosso do Sul state, in the Brazilian Cerrado region. Spleen and blood DNA samples were submitted to two conventional PCR protocols for Hepatozoon spp. based on 18S rRNA. No Hepatozoon spp. gamonts or meronts were observed in blood smears and HE-stained-liver preparations, respectively. While none of the spleen samples was positive for Hepatozoon spp. in the PCR assays, 5 (3 %) blood samples contained 18S rRNA Hepatozoon DNA, including 2/37 (5 %) Artibeus lituratus, 2/32 (6 %) A. planirostris, and 1/23 (4 %) Platyrrhinus lineatus. Out of 5 bats positive for Hepatozoon spp., 3 were parasitized by either Macronyssidae/Spinturnicidae mites or Streblidae flies. BLAST analysis showed that the sequences detected in bats had > 99 % identity with Hepatozoon sequences detected in amphibians and reptiles from Brazil, including Hepatozoon caimani detected in Caiman crocodiles. The phylogenetic inferences estimated by the Maximum Likelihood and Bayesian methods clustered the Hepatozoon sequences detected in Brazilian bats with those detected in reptiles and amphibians.en
dc.description.affiliationUniv Estadual Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Jaboticabal, Brazil
dc.description.affiliationUniv Catolica Dom Bosco, Lab Ciencias Ambientais & Sustentabilidade Agr, Campo Grande, MS, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Lab Ecol Mamiferos, Rio De Janeiro, Brazil
dc.description.affiliationUnespUniv Estadual Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Jaboticabal, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2018/02753-0
dc.description.sponsorshipIdFAPESP: 2019/15150-4
dc.description.sponsorshipIdFAPESP: 2019/13232-3
dc.description.sponsorshipIdFAPESP: 2017/14124-4
dc.description.sponsorshipIdFUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul): 59/300.187/2016
dc.description.sponsorshipIdCNPq: 302420/2017-7
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2020.101401
dc.identifier.citationTicks And Tick-borne Diseases. Munich: Elsevier Gmbh, v. 11, n. 3, 6 p., 2020.
dc.identifier.doi10.1016/j.ttbdis.2020.101401
dc.identifier.issn1877-959X
dc.identifier.urihttp://hdl.handle.net/11449/196739
dc.identifier.wosWOS:000522720000027
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTicks And Tick-borne Diseases
dc.sourceWeb of Science
dc.subjectChiroptera
dc.subjectHepatozoonosis
dc.subject18S rRNA
dc.titleMolecular detection of Hepatozoon spp. in non-hematophagous bats in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-0002-7743[2]

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