Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs

dc.contributor.authorChuffa, Luiz Gustavo de Almeida [UNESP]
dc.contributor.authorLupi Júnior, Luiz Antonio [UNESP]
dc.contributor.authorda Maia Lima, Alfredo Feio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:26:39Z
dc.date.available2018-12-11T17:26:39Z
dc.date.issued2015-09-10
dc.description.abstractIn Polycystic Ovaries (PCOs), the dynamics of sex hormone receptors and follicle-related apoptotic signaling remain unknown. In this study, we investigated the expression of androgen receptors (AR), estrogen receptors (ERα and ERβ), and apoptosis-related molecules (BAX, active caspase-3, Bcl-2 and Survivin) on different follicular stages of PCOs in adult dogs. Clinical evidences of high estradiol and testosterone levels, persistent estrus and vaginal discharge were observed. Inhibin B immunolabeling was increased in primary and 2 to 5-mm follicles, and a marked epithelial hyperplasia was common in the ovarian surface. Ovarian epithelia and primary follicles showed low expression of AR, ERα, and ERβ, whereas a moderate immunoexpression of AR was found in theca cells of secondary follicles and cysts. In PCOs, growing follicles displayed ERα expression, and secondary follicles exhibited higher ERβ expression. In addition, while few ERα-positive cells were found in the cysts, ERβ was moderately expressed in growing follicles and cysts. BAX was upregulated in the ovarian epithelium, primary follicles, and in the wall of follicular cysts. Active caspase-3 was significantly downregulated in the epithelium, primary follicles, and follicular cysts, whereas growing follicles had a strong immunoexpression in the granulosa cells. Bcl-2 and survivin were increased in the epithelium and primary follicles, and only survivin was upregulated in secondary and growing follicles. While Bcl-2 had a diffuse immunexpression in the follicular cysts, survivin was overexpressed by these cells. We concluded that sex steroid receptors and apoptotic proteins are differentially expressed in the follicles of adult dogs with PCOs.en
dc.description.affiliationDepartment of Anatomy Biosciences Institute UNESP – Univ. Estadual Paulista
dc.description.affiliationDepartment of Veterinary Faculty of Veterinary Medicine and Zootechny UNESP – Univ. Estadual Paulista
dc.description.affiliationUnespDepartment of Anatomy Biosciences Institute UNESP – Univ. Estadual Paulista
dc.description.affiliationUnespDepartment of Veterinary Faculty of Veterinary Medicine and Zootechny UNESP – Univ. Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent10-17
dc.identifierhttp://dx.doi.org/10.1016/j.tice.2015.12.003
dc.identifier.citationTissue and Cell, v. 48, n. 1, p. 10-17, 2015.
dc.identifier.doi10.1016/j.tice.2015.12.003
dc.identifier.file2-s2.0-84952900963.pdf
dc.identifier.issn1532-3072
dc.identifier.issn0040-8166
dc.identifier.lattes5121319676503034
dc.identifier.scopus2-s2.0-84952900963
dc.identifier.urihttp://hdl.handle.net/11449/177688
dc.language.isoeng
dc.relation.ispartofTissue and Cell
dc.relation.ispartofsjr0,410
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectApoptosis
dc.subjectAR
dc.subjectDog
dc.subjectER
dc.subjectPolycystic ovary
dc.subjectSex steroid receptors
dc.titleSex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogsen
dc.typeArtigo
unesp.author.lattes5121319676503034

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