Panax ginseng metabolite (GIM-1) modulates the effects of monobutyl phthalate (MBP) on the GPR30/GPER1 canonical pathway in human Sertoli cells

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de Freitas, André Teves A.G. [UNESP]
Pinho, Cristiane Figueiredo [UNESP]
Aquino, Ariana Musa [UNESP]
Domeniconi, Raquel Fantin [UNESP]
Justulin, Luis Antonio [UNESP]
Scarano, Wellerson Rodrigo [UNESP]

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This study was performed to evaluate the effect of monobutyl phthalate (MBP) on GPR30-activated pathways in Sertoli cells. Additionally, we tested if GIM-1 (Panax ginseng metabolite) modulates MBP action. Human Sertoli cells (HSeC lineage) were exposed to MBP and/or GIM-1 for 30 min, 1, 12, and 48 h. Four experimental treatments were performed: control (DEMEM/F12 medium), MBP, GIM-1, and MBP + GIM-1. The results indicate that MBP activates GPR30, PKA, Src, EGFR, and the ERK1/2 proteins, while GIM-1 inhibits PKA, Src, ERK1/2, and the AKT pathway. MBP also enhances Cofilin expression, decreasing F-actin intensity on the cell surface in a short time. The combined exposure demonstrated a functional antagonism between compounds. Collectively, these data show that MBP activates GPR30 in Sertoli cells, and GIM-1 modulates this response, playing a protective role in Sertoli cells exposed to MBP.



GIM-1, GPR30/GPER1, MBP, Panax ginseng, Sertoli cells

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Reproductive Toxicology, v. 96, p. 209-215.