Panax ginseng metabolite (GIM-1) modulates the effects of monobutyl phthalate (MBP) on the GPR30/GPER1 canonical pathway in human Sertoli cells
dc.contributor.author | de Freitas, André Teves A.G. [UNESP] | |
dc.contributor.author | Pinho, Cristiane Figueiredo [UNESP] | |
dc.contributor.author | Aquino, Ariana Musa [UNESP] | |
dc.contributor.author | Domeniconi, Raquel Fantin [UNESP] | |
dc.contributor.author | Justulin, Luis Antonio [UNESP] | |
dc.contributor.author | Scarano, Wellerson Rodrigo [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2020-12-12T02:46:32Z | |
dc.date.available | 2020-12-12T02:46:32Z | |
dc.date.issued | 2020-09-01 | |
dc.description.abstract | This study was performed to evaluate the effect of monobutyl phthalate (MBP) on GPR30-activated pathways in Sertoli cells. Additionally, we tested if GIM-1 (Panax ginseng metabolite) modulates MBP action. Human Sertoli cells (HSeC lineage) were exposed to MBP and/or GIM-1 for 30 min, 1, 12, and 48 h. Four experimental treatments were performed: control (DEMEM/F12 medium), MBP, GIM-1, and MBP + GIM-1. The results indicate that MBP activates GPR30, PKA, Src, EGFR, and the ERK1/2 proteins, while GIM-1 inhibits PKA, Src, ERK1/2, and the AKT pathway. MBP also enhances Cofilin expression, decreasing F-actin intensity on the cell surface in a short time. The combined exposure demonstrated a functional antagonism between compounds. Collectively, these data show that MBP activates GPR30 in Sertoli cells, and GIM-1 modulates this response, playing a protective role in Sertoli cells exposed to MBP. | en |
dc.description.affiliation | São Paulo State University (UNESP) Institute of Biosciences Department of Structural and Functional Biology | |
dc.description.affiliationUnesp | São Paulo State University (UNESP) Institute of Biosciences Department of Structural and Functional Biology | |
dc.format.extent | 209-215 | |
dc.identifier | http://dx.doi.org/10.1016/j.reprotox.2020.07.004 | |
dc.identifier.citation | Reproductive Toxicology, v. 96, p. 209-215. | |
dc.identifier.doi | 10.1016/j.reprotox.2020.07.004 | |
dc.identifier.issn | 1873-1708 | |
dc.identifier.issn | 0890-6238 | |
dc.identifier.lattes | 5481756528299469 | |
dc.identifier.orcid | 0000-0003-2938-010X | |
dc.identifier.scopus | 2-s2.0-85088628144 | |
dc.identifier.uri | http://hdl.handle.net/11449/201973 | |
dc.language.iso | eng | |
dc.relation.ispartof | Reproductive Toxicology | |
dc.source | Scopus | |
dc.subject | GIM-1 | |
dc.subject | GPR30/GPER1 | |
dc.subject | MBP | |
dc.subject | Panax ginseng | |
dc.subject | Sertoli cells | |
dc.title | Panax ginseng metabolite (GIM-1) modulates the effects of monobutyl phthalate (MBP) on the GPR30/GPER1 canonical pathway in human Sertoli cells | en |
dc.type | Artigo | |
unesp.author.lattes | 5481756528299469[4] | |
unesp.author.orcid | 0000-0002-0938-9505[1] | |
unesp.author.orcid | 0000-0002-6682-2934[6] | |
unesp.author.orcid | 0000-0003-2938-010X[4] |