Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

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dc.contributor.authorBernardino, Pedro Negri [UNESP]
dc.contributor.authorde Paula, Carolina Lechinski [UNESP]
dc.contributor.authorPereira, Ana Flávia Marques [UNESP]
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.authorde Carvalho Azevedo, Vasco Ariston
dc.contributor.authorBorges, Alexandre Secorun [UNESP]
dc.contributor.authorFernandes-Júnior, Ary [UNESP]
dc.contributor.authorOliveira-Filho, José Paes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of California – Davis
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2023-07-29T15:42:32Z
dc.date.available2023-07-29T15:42:32Z
dc.date.issued2023-03-01
dc.description.abstractCaseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.en
dc.description.affiliationSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationSchool of Veterinary Medicine Molecular Biosciences Department University of California – Davis
dc.description.affiliationInstitute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationGenetics Ecology and Evolution Department Federal University of Minas Gerais
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationUnespInstitute of Biosciences São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 132282/2019
dc.format.extent559-563
dc.identifierhttp://dx.doi.org/10.1007/s42770-022-00885-0
dc.identifier.citationBrazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023.
dc.identifier.doi10.1007/s42770-022-00885-0
dc.identifier.issn1678-4405
dc.identifier.issn1517-8382
dc.identifier.scopus2-s2.0-85144136740
dc.identifier.urihttp://hdl.handle.net/11449/249483
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subject2’,3’-Dideoxyadenosine
dc.subjectBactericidal
dc.subjectBacteriostatic
dc.subjectCaseous lymphadenitis
dc.subjectSmall ruminant
dc.titlePotential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosisen
dc.typeArtigo
unesp.author.orcid0000-0001-7770-6122[1]

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Artigos - Higiene Veterinária e Saúde Pública - FMVZ
Artigos - Clínica Veterinária - FMVZ