Zinc-Based Nanoparticles Reduce Bacterial Biofilm Formation

dc.contributor.authorFulindi, Rafael Bianchini [UNESP]
dc.contributor.authorRodrigues, Juliana Domingues [UNESP]
dc.contributor.authorBarbosa, Thulio Wliandon Lemos [UNESP]
dc.contributor.authorGarcia, Ariana D. Goncalves
dc.contributor.authorDe Almeida La Porta, Felipe
dc.contributor.authorPratavieira, Sebastião
dc.contributor.authorChiavacci, Leila Aparecida [UNESP]
dc.contributor.authorJunior, João Pessoa Araújo [UNESP]
dc.contributor.authorDa Costa, Paulo Inácio [UNESP]
dc.contributor.authorMartinez, Luis R.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Florida College of Dentistry
dc.contributor.institutionFederal Technological University of Paraná (UTFPR)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Florida
dc.date.accessioned2023-07-29T13:52:53Z
dc.date.available2023-07-29T13:52:53Z
dc.date.issued2023-03-01
dc.description.abstractBiofilm formation is important for microbial survival in hostile environments and a phenotype that provides microorganisms with antimicrobial resistance. Zinc oxide (ZnO) and Zinc sulfide (ZnS) nanoparticles (NPs) present potential antimicrobial properties for biomedical and food industry applications. Here, we aimed to analyze, for the first time, the bactericidal and antibiofilm activity of ZnS NPs against Staphylococcus aureus, Klebsiella oxytoca, and Pseudomonas aeruginosa, all medically important bacteria in developed countries. We compared ZnS NPs antimicrobial activity to ZnO NPs, which have been extensively studied. Using the colorimetric XTT reduction assay to observe the metabolic activity of bacterial cells and the crystal violet assay to measure biofilm mass, we demonstrated that ZnS and ZnO had similar efficacy in killing planktonic bacterial cells and reducing biofilm formation, with S. aureus being more susceptible to both therapeutics than K. oxytoca and P. aeruginosa. Crystal violet staining and confocal microscopy validated that Zn NPs inhibit biofilm formation and cause architectural damage. Our findings provide proof of principle that ZnS NPs have antibiofilm activity, and can be potentially used in medical and food industry applications, such as treatment of wound infections or package coating for food preservation. IMPORTANCE Zinc (Zn)-based nanoparticles (NPs) can be potentially used in medical and food preservation applications. As proof of principle, we investigated the bactericidal and antibiofilm activity of zinc oxide (ZnO) and zinc sulfide (ZnS) NPs against medically important bacteria. Zn-based NPs were similarly effective in killing planktonic and biofilm-Associated Staphylococcus aureus, Klebsiella oxytoca, and Pseudomonas aeruginosa cells. However, S. aureus was more susceptible to these investigational therapeutics. Although further studies are warranted, our findings suggest the possibility of future use of Zn-based NPs in the treatment of skin infections or preservation of food.en
dc.description.affiliationDepartments of Clinical Analysis São Paulo State University (UNESP), Araraquara
dc.description.affiliationDepartments of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara
dc.description.affiliationDepartment of Oral Biology University of Florida College of Dentistry
dc.description.affiliationDepartment of Chemistry Federal Technological University of Paraná (UTFPR), Londrina
dc.description.affiliationSão Carlos Physics Department University of São Paulo, São Carlos
dc.description.affiliationBiotechnology Institute São Paulo State University, Botucatu
dc.description.affiliationEmerging Pathogens Institute University of Florida
dc.description.affiliationCenter for Immunology and Transplantation University of Florida
dc.description.affiliationCenter for Translational Research in Neurodegenerative Disease University of Florida
dc.description.affiliationUnespDepartments of Clinical Analysis São Paulo State University (UNESP), Araraquara
dc.description.affiliationUnespDepartments of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara
dc.description.affiliationUnespBiotechnology Institute São Paulo State University, Botucatu
dc.identifierhttp://dx.doi.org/10.1128/spectrum.04831-22
dc.identifier.citationMicrobiology Spectrum, v. 11, n. 2, 2023.
dc.identifier.doi10.1128/spectrum.04831-22
dc.identifier.issn2165-0497
dc.identifier.scopus2-s2.0-85154035126
dc.identifier.urihttp://hdl.handle.net/11449/248756
dc.language.isoeng
dc.relation.ispartofMicrobiology Spectrum
dc.sourceScopus
dc.subjectantimicrobial activity
dc.subjectbacteria
dc.subjectbiofilms
dc.subjectnanoparticles
dc.subjectzinc oxide
dc.subjectzinc sulfide
dc.titleZinc-Based Nanoparticles Reduce Bacterial Biofilm Formationen
dc.typeArtigo

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