Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices

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dc.contributor.authorFernandes, Ellen Eduarda [UNESP]
dc.contributor.authorOliveira, Daniela Fernandes Lobo Molica [UNESP]
dc.contributor.authorJóias, Renata Pilli [UNESP]
dc.contributor.authorDiniz, Pamela Aparecida [UNESP]
dc.contributor.authorDe Barros, Patrícia Pimentel
dc.contributor.authorJorge, Antônio Olavo Cardoso [UNESP]
dc.contributor.authorDE Oliveira, Wagner [UNESP]
dc.contributor.authorRode, Sigmar De Mello [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte (UFRN)
dc.date.accessioned2023-07-29T13:54:22Z
dc.date.available2023-07-29T13:54:22Z
dc.date.issued2023-04-01
dc.description.abstractObjective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms.en
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SP
dc.description.affiliationEscola Multicampi de Ciências Médicas Universidade Federal do Rio Grande do Norte (UFRN), RN
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquita Filho” Instituto de Ciência e Tecnologia Departamento de Biociências e Diagnóstico Bucal, SP
dc.description.sponsorshipUniversidade Estadual Paulista
dc.identifierhttp://dx.doi.org/10.4322/bds.2023.e3440
dc.identifier.citationBrazilian Dental Science, v. 26, n. 2, 2023.
dc.identifier.doi10.4322/bds.2023.e3440
dc.identifier.issn2178-6011
dc.identifier.scopus2-s2.0-85159065638
dc.identifier.urihttp://hdl.handle.net/11449/248810
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Science
dc.sourceScopus
dc.subjectBiofilms
dc.subjectCandida albicans
dc.subjectOrthodontic appliances
dc.subjectOrthodontic brackets
dc.subjectScanning electron microscopy
dc.titleAnalysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devicesen
dc.titleAnálise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixospt
dc.typeArtigo

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