Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers

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dc.contributor.authorGabriel, J. E.
dc.contributor.authorFerro, J. A.
dc.contributor.authorStefani, RMP
dc.contributor.authorFerro, MIT
dc.contributor.authorGomes, S. L.
dc.contributor.authorMacari, Marcos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:21:58Z
dc.date.available2014-05-20T15:21:58Z
dc.date.issued1996-05-01
dc.description.abstract1. The synthesis of heat shock protein 70 (Hsp70) mRNA and the expression of Hsp70 in the liver of broiler chickens submitted to acute heat stress (35 degrees C for 5 h) was investigated.2. Hsp70 expression was detected by SDS-PAGE and Western blot analysis using a polyclonal antiserum against Hsp70 of Blastocladiella emersonii. The specific signal of Hsp70 mRNA was analysed by Northern blot using as probe a Hsp70 cDNA of B. emersonii.3. An increase in the amount of Hsp70 was detected from the first up to the fifth hour of acute heat exposure. This increase in the amount of Hsp70 was accompanied by an increase in Hsp70 mRNA which peaked at 3 h.4. This study shows that the heat induced increase in Hsp70 mRNA and protein in broiler liver, in vivo, are time dependent, similar to that in mammals.en
dc.description.affiliationUNIV ESTADUAL PAULISTA,FAC CIENCIAS AGR & VET,DEPT TECNOL,BR-14870000 JABOTICABAL,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA,FAC CIENCIAS AGR & VET,DEPT MORFOL & FISIOL ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL
dc.description.affiliationUNIV SAO PAULO,INST QUIM,DEPT BIOQUIM,BR-01498970 SAO PAULO,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,FAC CIENCIAS AGR & VET,DEPT TECNOL,BR-14870000 JABOTICABAL,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,FAC CIENCIAS AGR & VET,DEPT MORFOL & FISIOL ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL
dc.format.extent443-449
dc.identifierhttp://dx.doi.org/10.1080/00071669608417875
dc.identifier.citationBritish Poultry Science. Abingdon: Carfax Publ Co, v. 37, n. 2, p. 443-449, 1996.
dc.identifier.doi10.1080/00071669608417875
dc.identifier.issn0007-1668
dc.identifier.lattes0147241723612464
dc.identifier.lattes5713558572926669
dc.identifier.urihttp://hdl.handle.net/11449/33034
dc.identifier.wosWOS:A1996UM86100020
dc.language.isoeng
dc.publisherCarfax Publ Co
dc.relation.ispartofBritish Poultry Science
dc.relation.ispartofjcr1.096
dc.relation.ispartofsjr0,477
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEffect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilersen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderCarfax Publ Co
unesp.author.lattes0147241723612464
unesp.author.lattes5713558572926669
unesp.author.orcid0000-0002-6850-7145[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMorfologia e Fisiologia Animal - FCAVpt
unesp.departmentTecnologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias