Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
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Arquivos
Data
2015-05-01
Autores
Silva, Miguel Ladino
Ribeiro, Alexandre Pinto
Silva, Germana Alegro
Barbosa Sanchez, Irma Ximena
Renzo, Roberta
Uscategui, Ricardo
Lima, Tiago Barbalho
Aldrovani, Marcela
Laus, Jose Luiz
Título da Revista
ISSN da Revista
Título de Volume
Editor
Consel Brasil Oftalmologia
Resumo
Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.
Descrição
Palavras-chave
Cornea, Photorefractive keratectomy, Nalbuphine/therapeutic use, Matrix metalloproteinases, Receptors, opioid, Immunohistochemistry, Animals, Rabbits
Como citar
Arquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 78, n. 3, p. 141-145, 2015.