Writing 3D In Vitro Models of Human Tendon within a Biomimetic Fibrillar Support Platform

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dc.contributor.authorMonteiro, Rosa F.
dc.contributor.authorBakht, Syeda M.
dc.contributor.authorGomez-Florit, Manuel
dc.contributor.authorStievani, Fernanda C. [UNESP]
dc.contributor.authorAlves, Ana L. G. [UNESP]
dc.contributor.authorReis, Rui L.
dc.contributor.authorGomes, Manuela E.
dc.contributor.authorDomingues, Rui M. A.
dc.contributor.institutionHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine
dc.contributor.institutionICVS/3B’s─PT Government Associate Laboratory
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:48:31Z
dc.date.available2023-07-29T13:48:31Z
dc.date.issued2022-01-01
dc.description.abstractTendinopathies are poorly understood diseases for which treatment remains challenging. Relevant in vitro models to study human tendon physiology and pathophysiology are therefore highly needed. Here we propose the automated 3D writing of tendon microphysiological systems (MPSs) embedded in a biomimetic fibrillar support platform based on cellulose nanocrystals (CNCs) self-assembly. Tendon decellularized extracellular matrix (dECM) was used to formulate bioinks that closely recapitulate the biochemical signature of tendon niche. A monoculture system recreating the cellular patterns and phenotype of the tendon core was first developed and characterized. This system was then incorporated with a vascular compartment to study the crosstalk between the two cell populations. The combined biophysical and biochemical cues of the printed pattern and dECM hydrogel were revealed to be effective in inducing human-adipose-derived stem cells (hASCs) differentiation toward the tenogenic lineage. In the multicellular system, chemotactic effects promoted endothelial cells migration toward the direction of the tendon core compartment, while the established cellular crosstalk boosted hASCs tenogenesis, emulating the tendon development stages. Overall, the proposed concept is a promising strategy for the automated fabrication of humanized organotypic tendon-on-chip models that will be a valuable new tool for the study of tendon physiology and pathogenesis mechanisms and for testing new tendinopathy treatments.en
dc.description.affiliation3B’s Research Group I3Bs─Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco
dc.description.affiliationICVS/3B’s─PT Government Associate Laboratory
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction Regenerative Medicine Laboratory School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction Regenerative Medicine Laboratory School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1021/acsami.2c22371
dc.identifier.citationACS Applied Materials and Interfaces.
dc.identifier.doi10.1021/acsami.2c22371
dc.identifier.issn1944-8252
dc.identifier.issn1944-8244
dc.identifier.scopus2-s2.0-85151295674
dc.identifier.urihttp://hdl.handle.net/11449/248599
dc.language.isoeng
dc.relation.ispartofACS Applied Materials and Interfaces
dc.sourceScopus
dc.subjectcellulose nanocrystals
dc.subjectdecellularized extracellular matrix
dc.subjectmicrophysiological systems
dc.subjecttendinopathy
dc.subjecttendon-on-chip
dc.titleWriting 3D In Vitro Models of Human Tendon within a Biomimetic Fibrillar Support Platformen
dc.typeArtigo
unesp.author.orcid0000-0002-2036-6291 0000-0002-2036-6291[7]
unesp.author.orcid0000-0002-3654-9906 0000-0002-3654-9906[8]

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