Semen cryopreservation protocols of Mangalarga Marchador stallions

dc.contributor.authorCandeias, Marcela Leite
dc.contributor.authorAlvarenga, Marco Antonio [UNESP]
dc.contributor.authorCarmo, Márcio Teoro do [UNESP]
dc.contributor.authorFerreira, Heder Nunes [UNESP]
dc.contributor.authorMaior, Mônica Russo Souto
dc.contributor.authorTorres Filho, Rodolpho de Almeida
dc.contributor.authorRodrigues, André Luís Rios
dc.contributor.authorBrandão, Felipe Zandonadi
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T20:12:46Z
dc.date.accessioned2014-05-20T13:41:51Z
dc.date.available2013-09-30T20:12:46Z
dc.date.available2014-05-20T13:41:51Z
dc.date.issued2012-09-01
dc.description.abstractThe effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.en
dc.description.affiliationUniversidade Federal Fluminense (UFF) Faculdade de Veterinária
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e Zootecnia
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e Zootecnia
dc.format.extent1989-1995
dc.identifierhttp://dx.doi.org/10.1590/S1516-35982012000900004
dc.identifier.citationRevista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012.
dc.identifier.doi10.1590/S1516-35982012000900004
dc.identifier.fileS1516-35982012000900004.pdf
dc.identifier.issn1516-3598
dc.identifier.lattes0473846154288947
dc.identifier.scieloS1516-35982012000900004
dc.identifier.urihttp://hdl.handle.net/11449/14523
dc.identifier.wosWOS:000311562800004
dc.language.isoeng
dc.publisherSociedade Brasileira de Zootecnia
dc.relation.ispartofRevista Brasileira de Zootecnia
dc.relation.ispartofsjr0,337
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectejaculateden
dc.subjectequineen
dc.subjectextenderen
dc.subjectstrawen
dc.titleSemen cryopreservation protocols of Mangalarga Marchador stallionsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rbz/paboutj.htm
unesp.author.lattes0473846154288947
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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