Molecularly designed layer-by-layer (LbL) films to detect catechol using information visualization methods

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Aoki, Pedro H. B. [UNESP]
Alessio, Priscila [UNESP]
Furini, Leonardo N. [UNESP]
Constantino, Carlos J. L. [UNESP]
Neves, Tácito T. A. T.
Paulovich, Fernando V.
De Oliveira, Maria Cristina F.
Oliveira, Osvaldo N.

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The control of molecular architectures has been exploited in layer-by-layer (LbL) films deposited on Au interdigitated electrodes, thus forming an electronic tongue (e-tongue) system that reached an unprecedented high sensitivity (down to 10-12 M) in detecting catechol. Such high sensitivity was made possible upon using units containing the enzyme tyrosinase, which interacted specifically with catechol, and by processing impedance spectroscopy data with information visualization methods. These latter methods, including the parallel coordinates technique, were also useful for identifying the major contributors to the high distinguishing ability toward catechol. Among several film architectures tested, the most efficient had a tyrosinase layer deposited atop LbL films of alternating layers of dioctadecyldimethylammonium bromide (DODAB) and 1,2-dipalmitoyl-sn-3-glycero-fosfo-rac-(1-glycerol) (DPPG), viz., (DODAB/DPPG)5/DODAB/Tyr. The latter represents a more suitable medium for immobilizing tyrosinase when compared to conventional polyelectrolytes. Furthermore, the distinction was more effective at low frequencies where double-layer effects on the film/liquid sample dominate the electrical response. Because the optimization of film architectures based on information visualization is completely generic, the approach presented here may be extended to designing architectures for other types of applications in addition to sensing and biosensing. © 2013 American Chemical Society.



Dioctadecyl dimethylammonium bromides, Electrical response, Impedance spectroscopy, Information visualization, Inter-digitated electrodes, Layer-by-layer films, Molecular architecture, Parallel coordinates, Data handling, Deposits, Electronic tongues, Gold deposits, Information analysis, Information systems, Phenols, Gold

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Langmuir, v. 29, n. 24, p. 7542-7550, 2013.