A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract

Correa dos Santos, Carlos Henrique; Borges, Izabeau Pontes; Silva, Virginia Claudia da; Sousa, Paulo Teixeira de; Kawashita, Nair Honda; Baviera, Amanda Martins [UNESP]; Carvalho, Mario Geraldo de

A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract

Arquivos

WOS000382572400006.pdf (1.39 MB)

Data

2016-09-01

Autores

Correa dos Santos, Carlos Henrique

Borges, Izabeau Pontes

Silva, Virginia Claudia da

Sousa, Paulo Teixeira de

Kawashita, Nair Honda

Baviera, Amanda Martins

Carvalho, Mario Geraldo de

Editor

Taylor & Francis Ltd

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

ContextSiolmatra brasiliensis (Cogn.) Baill (Cucurbitaceae) is a climbing plant widely used for the treatment of diabetes mellitus symptoms.Objective This work evaluates the antidiabetic activity of an extract of S. brasiliensis in streptozotocin-diabetic rats and promotes the phytochemical investigation to isolate the major compounds of the same extract.Materials and methods Male Wistar rats were divided into normal (N) and diabetic rats (DC) treated with water; diabetic rats treated with 3U insulin (DI) or with 250 (DSb250) or 500mg/kg (DSb500) of hydroalcoholic extract of the stalks of S. brasiliensis, via oral gavage, for 21 days. Physiological and biochemical parameters classically altered in diabetes were monitored. The triterpenoids were isolated from the ethyl acetate fraction under silica gel column chromatography and Sephadex-LH20 methods and their structures were determined by NMR, HR-ESI-MS and DC analysis.Results When compared with DC, DSb250 rats showed a reduction in the hyperglycemia (DC: 26.460.69 versus DSb250: 19.67 +/- 1.06mmol/L) and glycosuria (DC: 43.02 +/- 3.19 versus DSb250: 28.46 +/- 2.14mmol/24h) and increase in hepatic glycogen (DC: 14.44 +/- 1.26 versus DSb250: 22.08 +/- 4.26mg/g). Three known cucurbitacins were isolated from a hydroalcoholic extract of S. brasiliensis, i.e., cayaponosides A1, B4, D, and a new dammarane saponin 3-O--d-gentiobiosyl-26-O--d-glucopyranosyl-20-hydroxydammar-24-ene. The structures of these compounds were elucidated by spectral data analysis of the natural products and their acetyl derivatives.Discussion and conclusion The known cucurbitacins and/or the new identified saponin may be related with the antidiabetic activity of S. brasiliensis.