Obesity influences the proteome of periodontal ligament tissues following periodontitis induction in rats

Página do item simplificado
dc.contributor.authorLopes, Maria Eduarda Scordamaia [UNESP]
dc.contributor.authorMarcantonio, Camila Chierici [UNESP]
dc.contributor.authorde Molon, Rafael Scaf [UNESP]
dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.authorSalmon, Cristiane Ribeiro
dc.contributor.authorMofatto, Luciana Souto
dc.contributor.authorNociti Junior, Francisco Humberto
dc.contributor.authorDeschner, James
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorNogueira, Andressa Vilas Boas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionSão Leopoldo Mandic Research Center
dc.contributor.institutionUniversity Medical Center of the Johannes Gutenberg University
dc.date.accessioned2022-04-29T08:40:16Z
dc.date.available2022-04-29T08:40:16Z
dc.date.issued2022-01-01
dc.description.abstractBackground and Objectives: Many studies have been conducted to better understand the molecular mechanism involved with periodontitis progression. There has been growing interest in the potential impact of obesity on periodontitis onset and progression, but the mechanisms involved remain to be elucidated. The present study was designed to determine the impact of obesity on experimentally induced periodontitis in rats and identify novel pathways involved. Methods: Sixteen Holtzman rats were distributed into two groups (n = 8): ligature-induced periodontitis (P) and obesity plus ligature-induced periodontitis (OP). Obesity was induced by a high-fat diet for 70 days, whereas periodontitis was induced for 20 days, with a cotton thread placed around the upper first molars bilaterally. Alveolar bone loss was measured by microtomographic analysis and histologically by histometry on the hemimaxillae. The protein composition of the periodontal ligament was evaluated by proteomic analysis. Results: Data analysis (body weight, adipose tissue weight, and blood test) confirmed obesity induction, whereas bone loss was confirmed by micro-CT and histologic analyses. Proteome analysis from the periodontal ligament tissues (PDL) identified 819 proteins, 53 exclusive to the P group, 28 exclusive to the OP group, and 738 commonly expressed. Validation was performed by immunohistochemistry for selected proteins (spondin1, vinculin, and TRAP). Conclusion: Histologically, it was found that obesity did not significantly affect bone loss resulting from periodontitis. However, the present study's findings indicated that obesity affects the proteome of PDL submitted to experimental periodontitis, allowing for identifying potential targets for personalized approaches.en
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University – UNESP
dc.description.affiliationDepartment of Morphology School of Dentistry at Araraquara São Paulo State University
dc.description.affiliationDivision of Periodontics Department of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas – UNICAMP
dc.description.affiliationDepartment of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas – UNICAMP
dc.description.affiliationSão Leopoldo Mandic Research Center
dc.description.affiliationDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University – UNESP
dc.description.affiliationUnespDepartment of Morphology School of Dentistry at Araraquara São Paulo State University
dc.identifierhttp://dx.doi.org/10.1111/jre.12983
dc.identifier.citationJournal of Periodontal Research.
dc.identifier.doi10.1111/jre.12983
dc.identifier.issn1600-0765
dc.identifier.issn0022-3484
dc.identifier.scopus2-s2.0-85125581684
dc.identifier.urihttp://hdl.handle.net/11449/230487
dc.language.isoeng
dc.relation.ispartofJournal of Periodontal Research
dc.sourceScopus
dc.subjectobesity
dc.subjectperiodontal disease
dc.subjectperiodontal ligament
dc.subjectperiodontitis
dc.subjectproteomics
dc.titleObesity influences the proteome of periodontal ligament tissues following periodontitis induction in ratsen
dc.typeArtigo
unesp.author.orcid0000-0003-4878-3174[1]
unesp.author.orcid0000-0002-0610-7074[2]
unesp.author.orcid0000-0003-1110-6233[3]
unesp.author.orcid0000-0001-5756-5828[4]
unesp.author.orcid0000-0003-3304-5064[5]
unesp.author.orcid0000-0002-2087-7251[6]
unesp.author.orcid0000-0001-6809-8866[7]
unesp.author.orcid0000-0002-7082-9290[9]
unesp.author.orcid0000-0002-2756-5947[10]

Arquivos

Coleções

Artigos - Diagnóstico e Cirurgia - FOAR
Artigos - Morfologia - FOAR