Molecular method confirms canine leishmania infection detected by serological methods in non-endemic area of Brazil

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dc.contributor.authorRiboldi, Emeline
dc.contributor.authorCarvalho, Flavio
dc.contributor.authorRomão, Pedro Roosevelt Torres
dc.contributor.authorBarcellos, Regina Bones
dc.contributor.authorBello, Graziele Lima
dc.contributor.authorRamos, Raquel Rocha
dc.contributor.authorde Oliveira, Rosemari Terezinha
dc.contributor.authorAraújo Júnior, João Pessoa [UNESP]
dc.contributor.authorRossetti, Maria Lucia
dc.contributor.authorDallegrave, Eliane
dc.contributor.institutionUniversidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)
dc.contributor.institutionUniversidade Luterana do Brasil (ULBRA)
dc.contributor.institutionFundação Estadual de Produção e Pesquisa em Saúde (FEPPS)
dc.contributor.institutionLaboratório Pathos
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:18:45Z
dc.date.available2018-12-11T17:18:45Z
dc.date.issued2018-02-01
dc.description.abstractIn Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n= 107), São Leopoldo (n= 216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic (DPP®) and ELISA EIE® assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in São Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.en
dc.description.affiliationUniversidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)
dc.description.affiliationUniversidade Luterana do Brasil (ULBRA)
dc.description.affiliationLaboratório de Imunologia Celular e Molecular Departamento de Ciências Básicas da Saúde Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)
dc.description.affiliationCentro de Desenvolvimento Científico Tecnológico (CDCT) Fundação Estadual de Produção e Pesquisa em Saúde (FEPPS)
dc.description.affiliationLaboratório Pathos
dc.description.affiliationDepartamento de Microbiologia e Imunologia Laboratório de Virologia/Diagnóstico Molecular Instituto de Biociências Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Laboratório de Virologia/Diagnóstico Molecular Instituto de Biociências Universidade Estadual Paulista (UNESP)
dc.format.extent11-19
dc.identifierhttp://dx.doi.org/10.3347/kjp.2018.56.1.11
dc.identifier.citationKorean Journal of Parasitology, v. 56, n. 1, p. 11-19, 2018.
dc.identifier.doi10.3347/kjp.2018.56.1.11
dc.identifier.issn1738-0006
dc.identifier.issn0023-4001
dc.identifier.scopus2-s2.0-85044355785
dc.identifier.urihttp://hdl.handle.net/11449/176063
dc.language.isoeng
dc.relation.ispartofKorean Journal of Parasitology
dc.relation.ispartofsjr0,660
dc.relation.ispartofsjr0,660
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDog
dc.subjectImmunoassay
dc.subjectLeishmania sp.
dc.subjectPrevalence
dc.subjectReal-time PCR
dc.titleMolecular method confirms canine leishmania infection detected by serological methods in non-endemic area of Brazilen
dc.typeArtigo

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Artigos - Microbiologia e Imunologia - IBB