Dose-responses of stem cells from human exfoliated teeth to infrared LED irradiation

dc.contributor.authorTurrioni, Ana Paula Silveira [UNESP]
dc.contributor.authorMontoro, Liege Aldrovandi [UNESP]
dc.contributor.authorBasso, Fernanda Gonçalves [UNESP]
dc.contributor.authorde Almeida, Leopoldina de Fátima Dantas [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:58:10Z
dc.date.available2018-12-11T16:58:10Z
dc.date.issued2015-01-01
dc.description.abstractDespite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (10<sup>4</sup> cells/cm<sup>2</sup>), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm<sup>2</sup>) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm<sup>2</sup> exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm<sup>2</sup>. It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm<sup>2</sup>.en
dc.description.affiliationDepartment of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, UNESP – Univ Estadual Paulista
dc.description.affiliationDepartment of Physiology and Pathology, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista
dc.description.affiliationDepartment of Restorative Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, UNESP – Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Physiology and Pathology, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Restorative Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 301291/2010-1
dc.format.extent409-415
dc.identifierhttp://dx.doi.org/10.1590/0103-6440201300148
dc.identifier.citationBrazilian Dental Journal, v. 26, n. 4, p. 409-415, 2015.
dc.identifier.doi10.1590/0103-6440201300148
dc.identifier.fileS0103-64402015000400409.pdf
dc.identifier.issn1806-4760
dc.identifier.issn0103-6440
dc.identifier.scieloS0103-64402015000400409
dc.identifier.scopus2-s2.0-84940045635
dc.identifier.urihttp://hdl.handle.net/11449/172020
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectInfrared
dc.subjectMetabolism
dc.subjectPhototherapy
dc.subjectPulp cells
dc.titleDose-responses of stem cells from human exfoliated teeth to infrared LED irradiationen
dc.typeArtigo
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-7455-6867[5]

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