A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

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dc.contributor.authorAngelini, Leticia Mara Lima [UNESP]
dc.contributor.authorSilva, Amanda Ribeiro Martins da [UNESP]
dc.contributor.authorRocco, Lucas de Freitas Coli [UNESP]
dc.contributor.authorMilagre, Cintia Duarte de Freitas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:12:28Z
dc.date.available2015-10-21T13:12:28Z
dc.date.issued2015-05-01
dc.description.abstractA modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.en
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio Claro
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Química Orgânica, Instituto de Química de Araraquara
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent113-116
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
dc.identifier.doi10.1590/S1517-838246120130851
dc.identifier.fileS1517-83822015000100113.pdf
dc.identifier.issn1517-8382
dc.identifier.lattes1425748916849376
dc.identifier.orcid0000-0001-5627-8616
dc.identifier.scieloS1517-83822015000100113
dc.identifier.urihttp://hdl.handle.net/11449/128702
dc.identifier.wosWOS:000355769900013
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectNitrile hydrataseen
dc.subjectAmidaseen
dc.subjectNitrilaseen
dc.subjectAmidase inhibitoren
dc.subjectHigh-throughput screeningen
dc.titleA high-throughput screening assay for distinguishing nitrile hydratases from nitrilasesen
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Microbiologia
unesp.author.lattes1425748916849376[4]
unesp.author.orcid0000-0001-5627-8616[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC
Artigos - Química Orgânica - IQ