A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
dc.contributor.author | Angelini, Leticia Mara Lima [UNESP] | |
dc.contributor.author | Silva, Amanda Ribeiro Martins da [UNESP] | |
dc.contributor.author | Rocco, Lucas de Freitas Coli [UNESP] | |
dc.contributor.author | Milagre, Cintia Duarte de Freitas [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2015-10-21T13:12:28Z | |
dc.date.available | 2015-10-21T13:12:28Z | |
dc.date.issued | 2015-05-01 | |
dc.description.abstract | A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. | en |
dc.description.affiliationUnesp | Universidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio Claro | |
dc.description.affiliationUnesp | Universidade Estadual Paulista, Departamento de Química Orgânica, Instituto de Química de Araraquara | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.format.extent | 113-116 | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en | |
dc.identifier.citation | Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015. | |
dc.identifier.doi | 10.1590/S1517-838246120130851 | |
dc.identifier.file | S1517-83822015000100113.pdf | |
dc.identifier.issn | 1517-8382 | |
dc.identifier.lattes | 1425748916849376 | |
dc.identifier.orcid | 0000-0001-5627-8616 | |
dc.identifier.scielo | S1517-83822015000100113 | |
dc.identifier.uri | http://hdl.handle.net/11449/128702 | |
dc.identifier.wos | WOS:000355769900013 | |
dc.language.iso | eng | |
dc.publisher | Soc Brasileira Microbiologia | |
dc.relation.ispartof | Brazilian Journal Of Microbiology | |
dc.relation.ispartofjcr | 1.810 | |
dc.relation.ispartofsjr | 0,630 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Nitrile hydratase | en |
dc.subject | Amidase | en |
dc.subject | Nitrilase | en |
dc.subject | Amidase inhibitor | en |
dc.subject | High-throughput screening | en |
dc.title | A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases | en |
dc.type | Artigo | |
dcterms.rightsHolder | Soc Brasileira Microbiologia | |
unesp.author.lattes | 1425748916849376[4] | |
unesp.author.orcid | 0000-0001-5627-8616[4] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Química, Araraquara | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claro | pt |
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