Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment
dc.contributor.author | Petrilli, R. | |
dc.contributor.author | Eloy, J. O. [UNESP] | |
dc.contributor.author | Paschoal, J. A.R. | |
dc.contributor.author | Lopez, R. F.V. | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2018-12-11T16:52:25Z | |
dc.date.available | 2018-12-11T16:52:25Z | |
dc.date.issued | 2018-01-01 | |
dc.description.abstract | 5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used. 5-FU was eluted at 5.2 min and thymine at 13.0 min. The method was selective, precise and accurate in the linear range from 0.3 to 6 μg/mL. The samples were stable after three cycles of freeze/thawing and extraction efficiency rates above 95% were obtained. In vitro skin penetration studies of an aqueous solution and a commercial cream of 5-FU were performed. The cream improved 5-FU retention into the skin and permeation through the skin compared to the solution. Therefore, the method developed herein can be applied to the study of formulations for topical delivery of 5-FU. | en |
dc.description.affiliation | School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo Ribeirao Preto, Avenida do Cafe s/n | |
dc.description.affiliation | School of Pharmaceutical Sciences UNESP Sao Paulo State University | |
dc.description.affiliationUnesp | School of Pharmaceutical Sciences UNESP Sao Paulo State University | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: #2013/15134-2 | |
dc.description.sponsorshipId | FAPESP: #2014/22451-7 | |
dc.description.sponsorshipId | FAPESP: 2012/23764-3 | |
dc.format.extent | 133-138 | |
dc.identifier | http://dx.doi.org/10.1691/ph.2018.7138 | |
dc.identifier.citation | Pharmazie, v. 73, n. 3, p. 133-138, 2018. | |
dc.identifier.doi | 10.1691/ph.2018.7138 | |
dc.identifier.issn | 0031-7144 | |
dc.identifier.scopus | 2-s2.0-85044123896 | |
dc.identifier.uri | http://hdl.handle.net/11449/170788 | |
dc.language.iso | eng | |
dc.relation.ispartof | Pharmazie | |
dc.relation.ispartofsjr | 0,375 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Scopus | |
dc.title | Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment | en |
dc.type | Artigo |