Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment

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dc.contributor.authorPetrilli, R.
dc.contributor.authorEloy, J. O. [UNESP]
dc.contributor.authorPaschoal, J. A.R.
dc.contributor.authorLopez, R. F.V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:52:25Z
dc.date.available2018-12-11T16:52:25Z
dc.date.issued2018-01-01
dc.description.abstract5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used. 5-FU was eluted at 5.2 min and thymine at 13.0 min. The method was selective, precise and accurate in the linear range from 0.3 to 6 μg/mL. The samples were stable after three cycles of freeze/thawing and extraction efficiency rates above 95% were obtained. In vitro skin penetration studies of an aqueous solution and a commercial cream of 5-FU were performed. The cream improved 5-FU retention into the skin and permeation through the skin compared to the solution. Therefore, the method developed herein can be applied to the study of formulations for topical delivery of 5-FU.en
dc.description.affiliationSchool of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo Ribeirao Preto, Avenida do Cafe s/n
dc.description.affiliationSchool of Pharmaceutical Sciences UNESP Sao Paulo State University
dc.description.affiliationUnespSchool of Pharmaceutical Sciences UNESP Sao Paulo State University
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2013/15134-2
dc.description.sponsorshipIdFAPESP: #2014/22451-7
dc.description.sponsorshipIdFAPESP: 2012/23764-3
dc.format.extent133-138
dc.identifierhttp://dx.doi.org/10.1691/ph.2018.7138
dc.identifier.citationPharmazie, v. 73, n. 3, p. 133-138, 2018.
dc.identifier.doi10.1691/ph.2018.7138
dc.identifier.issn0031-7144
dc.identifier.scopus2-s2.0-85044123896
dc.identifier.urihttp://hdl.handle.net/11449/170788
dc.language.isoeng
dc.relation.ispartofPharmazie
dc.relation.ispartofsjr0,375
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleQuantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatmenten
dc.typeArtigo

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