Biosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilms
dc.contributor.author | Bernardo, Wagner Luis de Carvalho [UNESP] | |
dc.contributor.author | Boriollo, Marcelo Fabiano Gomes | |
dc.contributor.author | Tonon, Caroline Coradi [UNESP] | |
dc.contributor.author | da Silva, Jeferson Júnior | |
dc.contributor.author | Oliveira, Mateus Cardoso | |
dc.contributor.author | de Moraes, Fernando Cruz | |
dc.contributor.author | Spolidorio, Denise Madalena Palomari [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade Federal de São Carlos (UFSCar) | |
dc.date.accessioned | 2023-07-29T14:52:29Z | |
dc.date.available | 2023-07-29T14:52:29Z | |
dc.date.issued | 2022-09-29 | |
dc.description.abstract | This study analyzed the antimicrobial and antibiofilm action and cytotoxicity of extract (HEScL) and silver nanoparticles (AgNPs-HEScL) from Syzygium cumini leaves. GC–MS, UV–Vis, EDX, FEG/SEM, DLS and zeta potential assays were used to characterize the extract or nanoparticles. Antimicrobial, antibiofilm and cytotoxicity analyses were carried out by in vitro methods: agar diffusion, microdilution and normal oral keratinocytes spontaneously immortalized (NOK-SI) cell culture. MICs of planktonic cells ranged from 31.2–250 (AgNPs-HEScL) to 1,296.8–10,375 μg/ml (HEScL) for Actinomyces naeslundii, Fusobacterium nucleatum, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus oralis, Veillonella dispar, and Candida albicans. AgNPs-HEScL showed antibiofilm effects (125–8,000 μg/ml) toward Candida albicans, Streptococcus mutans and Streptococcus oralis, and Staphylococcus aureus and Staphylococcus epidermidis. The NOK-SI exhibited no cytotoxicity when treated with 32.8 and 680.3 μg/ml of AgNPs-HEScL and HEScL, respectively, for 5 min. The data suggest potential antimicrobial and antibiofilm action of HEScL, and more specifically, AgNPs-HEScL, involving pathogens of medical and dental interest (dose-, time- and species-dependent). The cytotoxicity of HEScL and AgNPs-HEScL detected in NOK-SI was dose- and time-dependent. This study presents toxicological information about the lyophilized ethanolic extract of S. cumini leaves, including their metallic nanoparticles, and adds scientific values to incipient studies found in the literature. | en |
dc.description.affiliation | Department of Physiology and Pathology School of Dentistry São Paulo State University | |
dc.description.affiliation | Department of Oral Diagnosis Dental School of Piracicaba State University of Campinas | |
dc.description.affiliation | School of Chemistry Federal University of São Carlos | |
dc.description.affiliationUnesp | Department of Physiology and Pathology School of Dentistry São Paulo State University | |
dc.identifier | http://dx.doi.org/10.3389/fmicb.2022.995521 | |
dc.identifier.citation | Frontiers in Microbiology, v. 13. | |
dc.identifier.doi | 10.3389/fmicb.2022.995521 | |
dc.identifier.issn | 1664-302X | |
dc.identifier.scopus | 2-s2.0-85139873551 | |
dc.identifier.uri | http://hdl.handle.net/11449/249264 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Microbiology | |
dc.source | Scopus | |
dc.subject | antibiofilm activity | |
dc.subject | antimicrobial activity | |
dc.subject | normal oral keratinocytes spontaneously immortalized | |
dc.subject | odontogenic pathogens | |
dc.subject | silver nanoparticles | |
dc.subject | Syzygium cumini | |
dc.title | Biosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilms | en |
dc.type | Artigo |