Biosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilms

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dc.contributor.authorBernardo, Wagner Luis de Carvalho [UNESP]
dc.contributor.authorBoriollo, Marcelo Fabiano Gomes
dc.contributor.authorTonon, Caroline Coradi [UNESP]
dc.contributor.authorda Silva, Jeferson Júnior
dc.contributor.authorOliveira, Mateus Cardoso
dc.contributor.authorde Moraes, Fernando Cruz
dc.contributor.authorSpolidorio, Denise Madalena Palomari [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2023-07-29T14:52:29Z
dc.date.available2023-07-29T14:52:29Z
dc.date.issued2022-09-29
dc.description.abstractThis study analyzed the antimicrobial and antibiofilm action and cytotoxicity of extract (HEScL) and silver nanoparticles (AgNPs-HEScL) from Syzygium cumini leaves. GC–MS, UV–Vis, EDX, FEG/SEM, DLS and zeta potential assays were used to characterize the extract or nanoparticles. Antimicrobial, antibiofilm and cytotoxicity analyses were carried out by in vitro methods: agar diffusion, microdilution and normal oral keratinocytes spontaneously immortalized (NOK-SI) cell culture. MICs of planktonic cells ranged from 31.2–250 (AgNPs-HEScL) to 1,296.8–10,375 μg/ml (HEScL) for Actinomyces naeslundii, Fusobacterium nucleatum, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus oralis, Veillonella dispar, and Candida albicans. AgNPs-HEScL showed antibiofilm effects (125–8,000 μg/ml) toward Candida albicans, Streptococcus mutans and Streptococcus oralis, and Staphylococcus aureus and Staphylococcus epidermidis. The NOK-SI exhibited no cytotoxicity when treated with 32.8 and 680.3 μg/ml of AgNPs-HEScL and HEScL, respectively, for 5 min. The data suggest potential antimicrobial and antibiofilm action of HEScL, and more specifically, AgNPs-HEScL, involving pathogens of medical and dental interest (dose-, time- and species-dependent). The cytotoxicity of HEScL and AgNPs-HEScL detected in NOK-SI was dose- and time-dependent. This study presents toxicological information about the lyophilized ethanolic extract of S. cumini leaves, including their metallic nanoparticles, and adds scientific values to incipient studies found in the literature.en
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry São Paulo State University
dc.description.affiliationDepartment of Oral Diagnosis Dental School of Piracicaba State University of Campinas
dc.description.affiliationSchool of Chemistry Federal University of São Carlos
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry São Paulo State University
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2022.995521
dc.identifier.citationFrontiers in Microbiology, v. 13.
dc.identifier.doi10.3389/fmicb.2022.995521
dc.identifier.issn1664-302X
dc.identifier.scopus2-s2.0-85139873551
dc.identifier.urihttp://hdl.handle.net/11449/249264
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.sourceScopus
dc.subjectantibiofilm activity
dc.subjectantimicrobial activity
dc.subjectnormal oral keratinocytes spontaneously immortalized
dc.subjectodontogenic pathogens
dc.subjectsilver nanoparticles
dc.subjectSyzygium cumini
dc.titleBiosynthesis of silver nanoparticles from Syzygium cumini leaves and their potential effects on odontogenic pathogens and biofilmsen
dc.typeArtigo

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