Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection

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dc.contributor.authorEscobar, Taiane Acunha
dc.contributor.authorDöwich, Gabriela
dc.contributor.authorCantele, Leticia Carvalho
dc.contributor.authorGóss, Geórgia Camargo
dc.contributor.authorPorciúncula, Marcelo Lameiro
dc.contributor.authorLeite, Carla Teixeira [UNESP]
dc.contributor.authorZuravski, Luísa
dc.contributor.authorDuarte, Claudia Acosta
dc.contributor.authorLübeck, Irina
dc.contributor.institutionUNIPAMPA
dc.contributor.institutionURCAMP
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:10:43Z
dc.date.available2020-12-12T01:10:43Z
dc.date.issued2020-01-01
dc.description.abstractLeishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.en
dc.description.affiliationPrograma de Pós-Graduação em Bioquímica Universidade Federal do Pampa UNIPAMPA
dc.description.affiliationPrograma de Pós-Graduação em Ciência Animal UNIPAMPA
dc.description.affiliationGraduação em Medicina Veterinária UNIPAMPA
dc.description.affiliationCurso de Medicina Veterinária Universidade da Região da Campanha URCAMP
dc.description.affiliationMedicina Veterinária Programa de Pós-Graduação em Medicina Veterinária Universidade Estadual Paulista UNESP
dc.description.affiliationCurso de Graduação em Medicina Veterinária UNIPAMPA
dc.description.affiliationUnespMedicina Veterinária Programa de Pós-Graduação em Medicina Veterinária Universidade Estadual Paulista UNESP
dc.description.sponsorshipNorth Carolina Biotechnology Center
dc.description.sponsorshipUniversidade Federal do Rio Grande do Sul
dc.description.sponsorshipUniversidade Federal do Pampa
dc.format.extent199-211
dc.identifierhttp://dx.doi.org/10.5433/1679-0359.2020v41n1p199
dc.identifier.citationSemina:Ciencias Agrarias, v. 41, n. 1, p. 199-211, 2020.
dc.identifier.doi10.5433/1679-0359.2020v41n1p199
dc.identifier.issn1679-0359
dc.identifier.issn1676-546X
dc.identifier.scopus2-s2.0-85077389145
dc.identifier.urihttp://hdl.handle.net/11449/198363
dc.language.isoeng
dc.relation.ispartofSemina:Ciencias Agrarias
dc.sourceScopus
dc.subjectDiagnostic methods
dc.subjectHorses
dc.subjectInfection
dc.subjectLeishmania infantum
dc.titleApplications of polymerase chain reaction for the detection of equine Leishmania sp. Infectionen
dc.typeArtigo

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