Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil

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dc.contributor.authorColombo, Tatiana Elias [UNESP]
dc.contributor.authorVedovello, Danila
dc.contributor.authorAraki, Carlos Shigueru
dc.contributor.authorCogo-Moreira, Hugo
dc.contributor.authorPenha dos Santos, Izalco Nuremberg
dc.contributor.authorNegri Reis, Andreia Francesli
dc.contributor.authorCosta, Fabiana Rodrigues
dc.contributor.authorArao Antonio Cruz, Lilian Elisa
dc.contributor.authorCasagrande, Liane
dc.contributor.authorRegatieri, Livia Jose
dc.contributor.authorFerreira Junior, Jurandir
dc.contributor.authorMorais Bronzoni, Roberta Vieira de
dc.contributor.authorSchmidt, Diane J.
dc.contributor.authorNogueira, Mauricio Lacerda
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionPrefeitura Sao Jose do Rio Preto
dc.contributor.institutionUniv Fed Mato Grosso
dc.contributor.institutionTufts Univ
dc.date.accessioned2014-12-03T13:08:53Z
dc.date.available2014-12-03T13:08:53Z
dc.date.issued2013-12-01
dc.description.abstractBackground: Dengue is a serious public health problem in numerous countries. The ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.Objective: The present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio (R) Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).Study design: 220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.Results: In samples collected in a non-epidemic period we found a 100% agreement of ELISA and RTPCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p<0.05) in dengue negative samples.Conclusions: Due to false negative results for DENV-4, the sole use of the Panbio Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationFac Med Sao Jose do Rio Preto FAMERP, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio de Mesquitta & Filho, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Sao Paulo, Brazil
dc.description.affiliationPrefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso, Sinop, Mato Grosso, Brazil
dc.description.affiliationTufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA USA
dc.description.affiliationUnespUniv Estadual Paulista Julio de Mesquitta & Filho, Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 12/11733-6
dc.description.sponsorshipIdFAPESP: 11/10458-9
dc.format.extent710-712
dc.identifierhttp://dx.doi.org/10.1016/j.jcv.2013.10.021
dc.identifier.citationJournal Of Clinical Virology. Amsterdam: Elsevier Science Bv, v. 58, n. 4, p. 710-712, 2013.
dc.identifier.doi10.1016/j.jcv.2013.10.021
dc.identifier.issn1386-6532
dc.identifier.urihttp://hdl.handle.net/11449/111684
dc.identifier.wosWOS:000327820600019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Clinical Virology
dc.relation.ispartofjcr3.101
dc.relation.ispartofsjr1,609
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDengueen
dc.subjectNS1 Ag rapid assayen
dc.subjectFalse negative resultsen
dc.titleDengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-9411-9237[4]
unesp.author.orcid0000-0003-1102-2419[14]

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