Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing

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dc.contributor.authorOliveira, Maria Emilia Franco [UNESP]
dc.contributor.authorArrais, Aline Matos
dc.contributor.authorde Mello, Marco Roberto Bourg
dc.contributor.authorVergani, Gabriel Brun [UNESP]
dc.contributor.authorFigueira, Lucas Machado
dc.contributor.authorEsteves, Sérgio Novita
dc.contributor.authorPereira, Verônica Schinaider do Amaral
dc.contributor.authorGarcia, Alexandre Rossetto
dc.contributor.authorBartlewski, Pawel Mieczyslaw
dc.contributor.authorda Fonseca, Jeferson Ferreira
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Federal Rural do Rio de Janeiro
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversity of Guelph
dc.date.accessioned2022-04-28T19:49:32Z
dc.date.available2022-04-28T19:49:32Z
dc.date.issued2022-04-01
dc.description.abstractThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n = 21) or 12-day (G-12SOV, n = 21) progesterone (P4)-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p <.05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p >.05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p <.05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p >.05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4-device removal) was positively correlated (p <.05) with the number of CL (r =.95) and viable embryos (r =.91) in G-12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes.en
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista
dc.description.affiliationEmbrapa Caprinos e Ovinos
dc.description.affiliationDepartamento de Reprodução e Avaliação Animal Universidade Federal Rural do Rio de Janeiro
dc.description.affiliationFaculdade de Veterinária Universidade Federal Fluminense
dc.description.affiliationEmbrapa Pecuária Sudeste
dc.description.affiliationDepartment of Biomedical Sciences Ontario Veterinary College University of Guelph
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIdFAPEMIG: CVZ-PPM 00201-17
dc.format.extent393-401
dc.identifierhttp://dx.doi.org/10.1111/rda.14077
dc.identifier.citationReproduction in Domestic Animals, v. 57, n. 4, p. 393-401, 2022.
dc.identifier.doi10.1111/rda.14077
dc.identifier.issn1439-0531
dc.identifier.issn0936-6768
dc.identifier.scopus2-s2.0-85122747293
dc.identifier.urihttp://hdl.handle.net/11449/223243
dc.language.isoeng
dc.relation.ispartofReproduction in Domestic Animals
dc.sourceScopus
dc.subjectin vivo embryo production
dc.subjectnaturalized sheep breed
dc.subjectnon-surgical embryo recovery
dc.subjectNSER
dc.subjectsuperovulation
dc.titleStudy of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushingen
dc.typeArtigo
unesp.author.orcid0000-0002-7730-290X[1]
unesp.author.orcid0000-0003-3242-2294[5]
unesp.author.orcid0000-0002-3354-1474[8]
unesp.author.orcid0000-0003-2731-9137[9]
unesp.author.orcid0000-0001-8028-3984[10]

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