The selective neurotoxin DSP-4 impairs the noradrenergic projections from the locus coeruleus to the inferior colliculus in rats

dc.contributor.authorHormigo, Sebastian
dc.contributor.authorde Castro e Horta Junior, Jose de Anchieta [UNESP]
dc.contributor.authorGomez-Nieto, Ricardo
dc.contributor.authorLopez, Dolores E.
dc.contributor.institutionUniv Salamanca
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:34:09Z
dc.date.available2014-05-20T15:34:09Z
dc.date.issued2012-06-28
dc.description.abstractThe inferior colliculus (IC) and the locus coeruleus (LC) are two midbrain nuclei that integrate multimodal information and play a major role in novelty detection to elicit an orienting response. Despite the reciprocal connections between these two structures, the projection pattern and target areas of the LC within the subdivisions of the rat IC are still unknown. Here, we used tract-tracing approaches combined with immunohistochemistry, densitometry, and confocal microscopy (CM) analysis to describe a projection from the LC to the IC. Biotinylated dextran amine (BDA) injections into the LC showed that the LC-IC projection is mainly ipsilateral (90%) and reaches, to a major extent, the dorsal and lateral part of the IC and the intercollicular commissure. Additionally, some LC fibers extend into the central nucleus of the IC. The neurochemical nature of this projection is noradrenergic, given that tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DBH) colocalize with the BDA-Iabeled fibers from the LC. To determine the total field of the LC innervations in the IC, we destroyed the LC neurons and fibers using a highly selective neurotoxin, DSP-4, and then studied the distribution and density of TH- and DBH-immunolabeled axons in the IC. In the DSP-4 treated animals, the number of axonal fibers immunolabeled for TH and DBH were deeply decreased throughout the entire rostrocaudal extent of the IC and its subdivisions compared to controls. Our densitometry results showed that the IC receives up to 97% of its noradrenergic innervations from the LC neurons and only 3% from non-coeruleus neurons. Our results also indicate that TH immunoreactivity in the IC was less impaired than the immunoreactivity for DBH after DSP-4 administration. This is consistent with the existence of an important dopaminergic projection from the substantia nigra to the IC. In conclusion, our study demonstrates and quantifies the noradrenergic projection from the LC to the IC and its subdivisions. The re-examination of the TH and DBH immunoreactivity after DSP-4 treatment provides insights into the source, extent, and topographic distribution of the LC efferent network in the IC, and hence, contributes to our understanding of the role of the noradrenaline (NA) system in auditory processing.en
dc.description.affiliationUniv Salamanca, INCYL, Salamanca 37007, Spain
dc.description.affiliationUniv Salamanca, Inst Biomed Res Salamanca, Salamanca 37007, Spain
dc.description.affiliationSão Paulo State Univ UNESP, Biosci Inst, São Paulo, Brazil
dc.description.affiliationUniv Salamanca, Dept Cellular Biol & Pathol, Salamanca 37007, Spain
dc.description.affiliationUnespSão Paulo State Univ UNESP, Biosci Inst, São Paulo, Brazil
dc.description.sponsorshipSpanish Ministry of Science and Innovation (MICINN)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipAUIP (Programa de Becas de Movilidad Academica)
dc.description.sponsorshipIdMICINN: BFU2010-17754
dc.description.sponsorshipIdFAPESP: 08/02771-6
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.3389/fncir.2012.00041
dc.identifier.citationFrontiers In Neural Circuits. Lausanne: Frontiers Research Foundation, v. 6, p. 14, 2012.
dc.identifier.doi10.3389/fncir.2012.00041
dc.identifier.fileWOS000305800800001.pdf
dc.identifier.issn1662-5110
dc.identifier.urihttp://hdl.handle.net/11449/42441
dc.identifier.wosWOS:000305800800001
dc.language.isoeng
dc.publisherFrontiers Research Foundation
dc.relation.ispartofFrontiers In Neural Circuits
dc.relation.ispartofjcr3.131
dc.relation.ispartofsjr2,175
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectbiotinylated dextran amineen
dc.subjectdopamine beta hydroxylase deficiencyen
dc.subjectimmunohistochemistryen
dc.subjectdensitometryen
dc.subjectneurotoxicityen
dc.subjectnoradrenergic efferentsen
dc.subjectnoradrenergic modulationen
dc.subjecttyrosine hydroxylaseen
dc.titleThe selective neurotoxin DSP-4 impairs the noradrenergic projections from the locus coeruleus to the inferior colliculus in ratsen
dc.typeArtigo
dcterms.licensehttp://www.frontiersin.org/Copyright.aspx
dcterms.rightsHolderFrontiers Research Foundation
unesp.author.orcid0000-0003-3639-9861[2]
unesp.author.orcid0000-0002-1450-7246[4]
unesp.author.orcid0000-0003-3141-5965[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000305800800001.pdf
Tamanho:
2.63 MB
Formato:
Adobe Portable Document Format
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: