In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery
dc.contributor.author | de Souza, Jaqueline Brandão [UNESP] | |
dc.contributor.author | Rosa, Gustavo dos Santos [UNESP] | |
dc.contributor.author | Rossi, Mariana Correa [UNESP] | |
dc.contributor.author | Stievani, Fernanda de Castro [UNESP] | |
dc.contributor.author | Pfeifer, João Pedro Hübbe [UNESP] | |
dc.contributor.author | Krieck, André Massahiro Teramoto [UNESP] | |
dc.contributor.author | Bovolato, Ana Lívia de Carvalho [UNESP] | |
dc.contributor.author | Fonseca-Alves, Carlos Eduardo [UNESP] | |
dc.contributor.author | Borrás, Vicente Amigó | |
dc.contributor.author | Alves, Ana Liz Garcia [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Paulista University-UNIP | |
dc.contributor.institution | Universitat Politècnica de València | |
dc.date.accessioned | 2022-04-29T08:33:00Z | |
dc.date.available | 2022-04-29T08:33:00Z | |
dc.date.issued | 2021-08-27 | |
dc.description.abstract | Encapsulation of biological components in hydrogels is a well described method for controlled drug delivery of proteins, tissue engineering and intestinal colonization with beneficial bacteria. Given the potential of tissue engineering in clinical practice, this study aimed to evaluate the feasibility of encapsulation of adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate. We evaluated capsule morphology and cell viability, immunophenotype and release after encapsulation. Circular and irregular pores were observed on the hydrogel surface, in which MSCs were present and alive. Capsules demonstrated good capacity of absorption of liquid and cell viability was consistently high through the time points, indicating proper nutrient diffusion. Flow cytometry showed stability of stem cell surface markers, whereas immunohistochemistry revealed the expression of CD44 and absence of MHC-II through 7 days of culture. Stem cell encapsulation in sodium alginate hydrogel is a feasible technique that does not compromise cell viability and preserves their undifferentiated status, becoming a relevant option to further studies of tridimensional culture systems and in vivo bioactive agents delivery. | en |
dc.description.affiliation | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESP | |
dc.description.affiliation | Cell Engineering Lab Blood Transfusion Center Botucatu Medical School São Paulo State University UNESP | |
dc.description.affiliation | Institute of Health Sciences Paulista University-UNIP | |
dc.description.affiliation | Institut de Tecnologia de Materials Universitat Politècnica de València | |
dc.description.affiliationUnesp | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESP | |
dc.description.affiliationUnesp | Cell Engineering Lab Blood Transfusion Center Botucatu Medical School São Paulo State University UNESP | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | CAPES: 001 | |
dc.description.sponsorshipId | CNPq: 142322/2016-4 GD | |
dc.description.sponsorshipId | FAPESP: 2017/14460–4 2017/12815-0 | |
dc.identifier | http://dx.doi.org/10.3389/fbioe.2021.674581 | |
dc.identifier.citation | Frontiers in Bioengineering and Biotechnology, v. 9. | |
dc.identifier.doi | 10.3389/fbioe.2021.674581 | |
dc.identifier.issn | 2296-4185 | |
dc.identifier.scopus | 2-s2.0-85114731341 | |
dc.identifier.uri | http://hdl.handle.net/11449/229505 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Bioengineering and Biotechnology | |
dc.source | Scopus | |
dc.subject | alginic acid | |
dc.subject | biocompatibility | |
dc.subject | biomaterial | |
dc.subject | cell encapsulation | |
dc.subject | tridimensional culture | |
dc.title | In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery | en |
dc.type | Artigo | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
unesp.department | Reprodução Animal e Radiologia Veterinária - FMVZ | pt |