In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery

dc.contributor.authorde Souza, Jaqueline Brandão [UNESP]
dc.contributor.authorRosa, Gustavo dos Santos [UNESP]
dc.contributor.authorRossi, Mariana Correa [UNESP]
dc.contributor.authorStievani, Fernanda de Castro [UNESP]
dc.contributor.authorPfeifer, João Pedro Hübbe [UNESP]
dc.contributor.authorKrieck, André Massahiro Teramoto [UNESP]
dc.contributor.authorBovolato, Ana Lívia de Carvalho [UNESP]
dc.contributor.authorFonseca-Alves, Carlos Eduardo [UNESP]
dc.contributor.authorBorrás, Vicente Amigó
dc.contributor.authorAlves, Ana Liz Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPaulista University-UNIP
dc.contributor.institutionUniversitat Politècnica de València
dc.date.accessioned2022-04-29T08:33:00Z
dc.date.available2022-04-29T08:33:00Z
dc.date.issued2021-08-27
dc.description.abstractEncapsulation of biological components in hydrogels is a well described method for controlled drug delivery of proteins, tissue engineering and intestinal colonization with beneficial bacteria. Given the potential of tissue engineering in clinical practice, this study aimed to evaluate the feasibility of encapsulation of adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate. We evaluated capsule morphology and cell viability, immunophenotype and release after encapsulation. Circular and irregular pores were observed on the hydrogel surface, in which MSCs were present and alive. Capsules demonstrated good capacity of absorption of liquid and cell viability was consistently high through the time points, indicating proper nutrient diffusion. Flow cytometry showed stability of stem cell surface markers, whereas immunohistochemistry revealed the expression of CD44 and absence of MHC-II through 7 days of culture. Stem cell encapsulation in sodium alginate hydrogel is a feasible technique that does not compromise cell viability and preserves their undifferentiated status, becoming a relevant option to further studies of tridimensional culture systems and in vivo bioactive agents delivery.en
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESP
dc.description.affiliationCell Engineering Lab Blood Transfusion Center Botucatu Medical School São Paulo State University UNESP
dc.description.affiliationInstitute of Health Sciences Paulista University-UNIP
dc.description.affiliationInstitut de Tecnologia de Materials Universitat Politècnica de València
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESP
dc.description.affiliationUnespCell Engineering Lab Blood Transfusion Center Botucatu Medical School São Paulo State University UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 142322/2016-4 GD
dc.description.sponsorshipIdFAPESP: 2017/14460–4 2017/12815-0
dc.identifierhttp://dx.doi.org/10.3389/fbioe.2021.674581
dc.identifier.citationFrontiers in Bioengineering and Biotechnology, v. 9.
dc.identifier.doi10.3389/fbioe.2021.674581
dc.identifier.issn2296-4185
dc.identifier.scopus2-s2.0-85114731341
dc.identifier.urihttp://hdl.handle.net/11449/229505
dc.language.isoeng
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.sourceScopus
dc.subjectalginic acid
dc.subjectbiocompatibility
dc.subjectbiomaterial
dc.subjectcell encapsulation
dc.subjecttridimensional culture
dc.titleIn Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Deliveryen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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