The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model

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dc.contributor.authorMeccatti, Vanessa Marques [UNESP]
dc.contributor.authorFigueiredo-Godoi, Lívia Mara Alves [UNESP]
dc.contributor.authorPereira, Thaís Cristine [UNESP]
dc.contributor.authorde Lima, Patrícia Michelle Nagai [UNESP]
dc.contributor.authorAbu Hasna, Amjad [UNESP]
dc.contributor.authorSenna, Lavinia Barbosa [UNESP]
dc.contributor.authorMarcucci, Maria Cristina [UNESP]
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorde Oliveira, Luciane Dias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T14:04:05Z
dc.date.available2023-07-29T14:04:05Z
dc.date.issued2022-12-01
dc.description.abstractThis study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan–Meier method and statistical analysis was performed using the log-rank test (Mantel–Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP
dc.description.affiliationDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), Av. Eng. Francisco José Longo Avenue 777, SP
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP
dc.description.affiliationUnespDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), Av. Eng. Francisco José Longo Avenue 777, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 15/25338-0
dc.identifierhttp://dx.doi.org/10.1038/s41598-022-19425-9
dc.identifier.citationScientific Reports, v. 12, n. 1, 2022.
dc.identifier.doi10.1038/s41598-022-19425-9
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85138189776
dc.identifier.urihttp://hdl.handle.net/11449/249159
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.sourceScopus
dc.titleThe biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella modelen
dc.typeArtigo
unesp.author.orcid0000-0002-1112-985X[5]

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Artigos - Biociências e Diagnóstico Bucal - ICT