Effect of low-level laser therapy in an experimental model of osteoarthritis in rats evaluated through raman spectroscopy

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dc.contributor.authorMangueira, Nilton Maciel
dc.contributor.authorXavier, Murilo
dc.contributor.authorSouza, Renato Aparecido de
dc.contributor.authorCastillo Salgado, Miguel Angel [UNESP]
dc.contributor.authorSilveira, Landulfo
dc.contributor.authorVillaverde, Antonio Balbin
dc.contributor.institutionUniversidade Federal do Piauí (UFPI)
dc.contributor.institutionUniversidade Federal do Vale do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.institutionInstituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais (IFSULDEMINAS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Camilo Castelo Branco (UNICASTELO)
dc.date.accessioned2015-10-22T06:40:17Z
dc.date.available2015-10-22T06:40:17Z
dc.date.issued2015-03-01
dc.description.abstractObjective: This work aimed to investigate the biochemical changes associated with low-level laser therapy (LLLT) using 660 and 780 nm, on a well-established experimental model of osteoarthritis (OA) in the knees of rats with induced collagenase, using histomorphometry and Raman spectroscopy. Materials and methods: Thirty-six Wistar rats were divided into four groups: control (GCON, n=9), collagenase without treatment (GCOL, n=9), collagenase with LLLT 660 nm treatment (G660, n=8), and collagenase with LLLT 780 nm treatment (G780, n=10). LLLT protocol was: 30 mW power output, 10 sec irradiation time, 0.04 cm(2) spot size, 0.3 J energy, 0.75 W/cm(2) irradiance, and 7.5 J/cm(2) fluence per session per day, during 14 days. Then, knees were withdrawn and submitted to histomorphometry and Raman spectroscopy analysis. Principal components analysis (PCA) and Mahalanobis distance were employed to characterize the spectral findings. Results: Histomorphometry revealed a significant increase in the amount of collagen III for the group irradiated with 660 nm. The Raman bands at 1247, 1273, and 1453 cm(-1) (from principal component score PC2), attributed to collagen type II, and 1460 cm(-1) (from PC3), attributed to collagen type III, suggested that the LLLT causes acceleration in cellular activity, especially on the cells that repair cartilage, accelerating the breakdown of cartilage destroyed by collagenase and stimulating the fibroblast to synthesize repairing collagen III. Conclusions: LLLT accelerated the initial breakdown of cartilage destroyed by collagenase and stimulated the fibroblast to synthesize the repairing collagen III, suggesting a beneficial effect of LLLT on OA.en
dc.description.affiliationUniv Fed Piaui, Dept Physiotherapy, Teresina, PI, Brazil
dc.description.affiliationUniv Fed Vale Jequitinhonha &Mucuri, Dept Physiotherapy, Unai, MG, Brazil
dc.description.affiliationInst Fed Educ Ciencia &Tecnol Sul Minas Gerais, Juiz De Fora, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biociencias &Diagnost Bucal, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Camilo Castelo Branco, Inst Biomed Engn, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biociencias & Diagnost Bucal, Sao Paulo, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPEMIG: APQ-02900-10
dc.description.sponsorshipIdFAPEMIG: APQ-01733-11
dc.description.sponsorshipIdFAPESP: 2009/01788-5
dc.format.extent145-153
dc.identifierhttp://online.liebertpub.com/doi/10.1089/pho.2014.3744
dc.identifier.citationPhotomedicine And Laser Surgery, v. 33, n. 3, p. 145-153, 2015.
dc.identifier.doi10.1089/pho.2014.3744
dc.identifier.issn1549-5418
dc.identifier.lattes8235700830441386
dc.identifier.urihttp://hdl.handle.net/11449/129736
dc.identifier.wosWOS:000350550700005
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofPhotomedicine And Laser Surgery
dc.relation.ispartofjcr1.620
dc.relation.ispartofsjr0,443
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEffect of low-level laser therapy in an experimental model of osteoarthritis in rats evaluated through raman spectroscopyen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.author.lattes8235700830441386
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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