Changes in gene expression in PBMCs profiles of PPAR alpha target genes in obese and non-obese individuals during fasting

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dc.contributor.authorFelicidade, Ingrid [UNESP]
dc.contributor.authorMarcarini, Juliana Cristina [UNESP]
dc.contributor.authorCarreira, Clisia Mara
dc.contributor.authorAmarante, Marla Karine
dc.contributor.authorAfrnan, Lydia A.
dc.contributor.authorMantoyani, Mario Sergio
dc.contributor.authorRibeiro, Lucia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionWageningen University (WUR)
dc.date.accessioned2015-10-21T13:09:29Z
dc.date.available2015-10-21T13:09:29Z
dc.date.issued2015-01-01
dc.description.abstractBackground: The prevalence of obesity has risen dramatically and the World Health Organization estimates that 700 million people will be obese worldwide by 2015. Approximately, 50% of the Brazilian population above 20 years of age is overweight, and 16% is obese. Aim: This study aimed to evaluate the differences in the expression of PPAR alpha target genes in human peripheral blood mononuclear cells (PBMCs) and free fatty acids (FFA) in obese and non-obese individuals after 24 h of fasting. We first presented evidence that Brazilian people exhibit expression changes in PPARa target genes in PBMCs under fasting conditions. Methods: Q-PCR was utilized to assess the mRNA expression levels of target genes. Results: In both groups, the FFA concentrations in. creased significantly after 24 h of fasting. The basal FFA mean concentration was two-fold higher in the obese group compared with the non-obese group. After fasting, all genes evaluated in this study showed increased expression levels compared with basal expression in both groups. Conclusion: However, our results reveal no differences in gene expression between the obese and non-obese, more studies are necessary to precisely delineate the associated mechanisms, particularly those that include groups with different degrees of obesity and patients with diabetes mellitus type 2 because the expression of the main genes that are involved in beta-oxidation and glucose level maintenance are affected by these factors.en
dc.description.affiliationUniversidade Estadual de Londrina, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde
dc.description.affiliationUniversidade Estadual de Londrina, Departamento de Patologia, Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde
dc.description.affiliationUniversidade Estadual de Londrina, Departamento de Biologia Geral, Centro de Ciências Biológicas
dc.description.affiliationWageningen University, Nutrition, Metabolism, and Genomics Group, Division of Human Nutrition
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Patologia, Faculdade de Medicina de Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio Claro
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent19-25
dc.identifierhttp://www.karger.com/Article/FullText/367668
dc.identifier.citationAnnals Of Nutrition And Metabolism. Basel: Karger, v. 66, n. 1, p. 19-25, 2015.
dc.identifier.doi10.1159/000367668
dc.identifier.issn0250-6807
dc.identifier.urihttp://hdl.handle.net/11449/128391
dc.identifier.wosWOS:000349659900003
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofAnnals Of Nutrition And Metabolism
dc.relation.ispartofjcr3.051
dc.relation.ispartofsjr1,317
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFree fatty acidsen
dc.subjectObesityen
dc.subjectPBMCen
dc.subjectPPAR alphaen
dc.titleChanges in gene expression in PBMCs profiles of PPAR alpha target genes in obese and non-obese individuals during fastingen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.orcid0000-0001-8218-8518[4]
unesp.author.orcid0000-0001-8981-2440[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Patologia - FMB
Artigos - Biologia - IBRC